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1fnq

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(New page: 200px<br /><applet load="1fnq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fnq, resolution 2.60&Aring;" /> '''CRYSTAL STRUCTURE AN...)
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[[Image:1fnq.gif|left|200px]]<br /><applet load="1fnq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fnq, resolution 2.60&Aring;" />
 
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'''CRYSTAL STRUCTURE ANALYSIS OF THE MUTANT REACTION CENTER PRO L209-> GLU FROM THE PHOTOSYNTHETIC PURPLE BACTERIUM RHODOBACTER SPHAEROIDES'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE ANALYSIS OF THE MUTANT REACTION CENTER PRO L209-> GLU FROM THE PHOTOSYNTHETIC PURPLE BACTERIUM RHODOBACTER SPHAEROIDES==
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The structures of the reaction center variants Pro L209 --&gt; Tyr, Pro L209, --&gt; Phe, and Pro L209 --&gt; Glu from the photosynthetic purple bacterium, Rhodobacter sphaeroides have been determined by X-ray crystallography to, 2.6-2.8 A resolution. These variants were constructed to interrupt a chain, of tightly bound water molecules that was assumed to facilitate proton, transfer from the cytoplasm to the secondary quinone Q(B) [Baciou, L., and, Michel, H. (1995) Biochemistry 34, 7967-7972]. However, the amino acid, exchanges Pro L209 --&gt; Tyr and Pro L209 --&gt; Phe do not interrupt the water, chain. Both aromatic side chains are oriented away from this water chain, and interact with three surrounding polar side chains (Asp L213, Thr L226, and Glu H173) which are displaced by up to 2.6 A. The conformational, changes induced by the bulky aromatic rings of Tyr L209 and Phe L209 lead, to unexpected displacements of Q(B) compared to the wild-type protein. In, the structure of the Pro L209 --&gt; Tyr variant, Q(B) is shifted by, approximately 4 A and is now located at a position similar to that, reported for the wild-type reaction center after illumination [Stowell, M., H. B., et al. (1997) Science 276, 812-816]. In the Pro L209 --&gt; Phe, variant, the electron density map reveals an intermediate Q(B) position, between the binding sites of the wild-type protein in the dark and the Pro, L209 --&gt; Tyr protein. In the Pro L209 --&gt; Glu reaction center, the, carboxylic side chain of Glu L209 is located within the water chain, and, the binding site of Q(B) remains unchanged compared to the wild-type, structure.
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<StructureSection load='1fnq' size='340' side='right'caption='[[1fnq]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1fnq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FNQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FNQ FirstGlance]. <br>
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1FNQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with FE, PO4, BCL, BPH, U10, SPO and LDA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FNQ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SPO:SPHEROIDENE'>SPO</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fnq OCA], [https://pdbe.org/1fnq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fnq RCSB], [https://www.ebi.ac.uk/pdbsum/1fnq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fnq ProSAT]</span></td></tr>
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X-ray structure analyses of photosynthetic reaction center variants from Rhodobacter sphaeroides: structural changes induced by point mutations at position L209 modulate electron and proton transfer., Kuglstatter A, Ermler U, Michel H, Baciou L, Fritzsch G, Biochemistry. 2001 Apr 10;40(14):4253-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11284681 11284681]
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</table>
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[[Category: Protein complex]]
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== Function ==
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[[Category: Rhodobacter sphaeroides]]
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[https://www.uniprot.org/uniprot/RCEL_CERSP RCEL_CERSP] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
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[[Category: Baciou, L.]]
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== Evolutionary Conservation ==
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[[Category: Ermler, U.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Fritzsch, G.]]
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Check<jmol>
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[[Category: Kuglstatter, A.]]
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<jmolCheckbox>
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[[Category: Michel, H.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fn/1fnq_consurf.spt"</scriptWhenChecked>
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[[Category: BCL]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: BPH]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: FE]]
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</jmolCheckbox>
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[[Category: LDA]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fnq ConSurf].
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[[Category: PO4]]
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<div style="clear:both"></div>
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[[Category: SPO]]
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__TOC__
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[[Category: U10]]
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</StructureSection>
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[[Category: interruption of water chain]]
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[[Category: Cereibacter sphaeroides]]
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[[Category: Large Structures]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:04:21 2007''
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[[Category: Baciou L]]
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[[Category: Ermler U]]
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[[Category: Fritzsch G]]
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[[Category: Kuglstatter A]]
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[[Category: Michel H]]

Current revision

CRYSTAL STRUCTURE ANALYSIS OF THE MUTANT REACTION CENTER PRO L209-> GLU FROM THE PHOTOSYNTHETIC PURPLE BACTERIUM RHODOBACTER SPHAEROIDES

PDB ID 1fnq

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