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1g28

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[[Image:1g28.gif|left|200px]]
 
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{{Structure
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==STRUCTURE OF A FLAVIN-BINDING DOMAIN, LOV2, FROM THE CHIMERIC PHYTOCHROME/PHOTOTROPIN PHOTORECEPTOR PHY3==
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|PDB= 1g28 |SIZE=350|CAPTION= <scene name='initialview01'>1g28</scene>, resolution 2.73&Aring;
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<StructureSection load='1g28' size='340' side='right'caption='[[1g28]], [[Resolution|resolution]] 2.73&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FMN:FLAVIN MONONUCLEOTIDE'>FMN</scene>
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<table><tr><td colspan='2'>[[1g28]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Adiantum_capillus-veneris Adiantum capillus-veneris]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G28 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.73&#8491;</td></tr>
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|GENE= PHY3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2759 Eukaryota])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g28 OCA], [https://pdbe.org/1g28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g28 RCSB], [https://www.ebi.ac.uk/pdbsum/1g28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g28 ProSAT]</span></td></tr>
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</table>
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'''STRUCTURE OF A FLAVIN-BINDING DOMAIN, LOV2, FROM THE CHIMERIC PHYTOCHROME/PHOTOTROPIN PHOTORECEPTOR PHY3'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q9ZWQ6_ADICA Q9ZWQ6_ADICA]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Phototropin, a major blue-light receptor for phototropism in seed plants, exhibits blue-light-dependent autophosphorylation and contains two light, oxygen, or voltage (LOV) domains and a serine/threonine kinase domain. The LOV domains share homology with the PER-ARNT-SIM (PAS) superfamily, a diverse group of sensor proteins. Each LOV domain noncovalently binds a single FMN molecule and exhibits reversible photochemistry in vitro when expressed separately or in tandem. We have determined the crystal structure of the LOV2 domain from the phototropin segment of the chimeric fern photoreceptor phy3 to 2.7-A resolution. The structure constitutes an FMN-binding fold that reveals how the flavin cofactor is embedded in the protein. The single LOV2 cysteine residue is located 4.2 A from flavin atom C(4a), consistent with a model in which absorption of blue light induces formation of a covalent cysteinyl-C(4a) adduct. Residues that interact with FMN in the phototropin segment of the chimeric fern photoreceptor (phy3) LOV2 are conserved in LOV domains from phototropin of other plant species and from three proteins involved in the regulation of circadian rhythms in Arabidopsis and Neurospora. This conservation suggests that these domains exhibit the same overall fold and share a common mechanism for flavin binding and light-induced signaling.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g2/1g28_consurf.spt"</scriptWhenChecked>
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1G28 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G28 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of a flavin-binding plant photoreceptor domain: insights into light-mediated signal transduction., Crosson S, Moffat K, Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):2995-3000. Epub 2001 Feb 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11248020 11248020]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g28 ConSurf].
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[[Category: Eukaryota]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Crosson, S.]]
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</StructureSection>
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[[Category: Moffat, K.]]
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[[Category: Adiantum capillus-veneris]]
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[[Category: FMN]]
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[[Category: Large Structures]]
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[[Category: alpha-beta structure]]
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[[Category: Phototropin]]
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[[Category: flavoprotein]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: fmn-binding domain]]
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[[Category: Crosson S]]
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[[Category: lov]]
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[[Category: Moffat K]]
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[[Category: pas fold]]
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[[Category: photoreceptor]]
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[[Category: phototropin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:17:11 2008''
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Current revision

STRUCTURE OF A FLAVIN-BINDING DOMAIN, LOV2, FROM THE CHIMERIC PHYTOCHROME/PHOTOTROPIN PHOTORECEPTOR PHY3

PDB ID 1g28

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