1g5c

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(New page: 200px<br /><applet load="1g5c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g5c, resolution 2.1&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1g5c.jpg|left|200px]]<br /><applet load="1g5c" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g5c, resolution 2.1&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE 'CAB' TYPE BETA CLASS CARBONIC ANHYDRASE FROM METHANOBACTERIUM THERMOAUTOTROPHICUM'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE 'CAB' TYPE BETA CLASS CARBONIC ANHYDRASE FROM METHANOBACTERIUM THERMOAUTOTROPHICUM==
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The structure of the "cab"-type beta class carbonic anhydrase from the, archaeon Methanobacterium thermoautotrophicum (Cab) has been determined to, 2.1-A resolution using the multiwavelength anomalous diffraction phasing, technique. Cab exists as a dimer with a subunit fold similar to that, observed in "plant"-type beta class carbonic anhydrases. The active site, zinc is coordinated by protein ligands Cys(32), His(87), and Cys(90), with, the tetrahedral coordination completed by a water molecule. The major, difference between plant- and cab-type beta class carbonic anhydrases is, in the organization of the hydrophobic pocket. The structure reveals a, Hepes buffer molecule bound 8 A away from the active site zinc, which, suggests a possible proton transfer pathway from the active site to the, solvent.
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<StructureSection load='1g5c' size='340' side='right'caption='[[1g5c]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1g5c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G5C FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5c OCA], [https://pdbe.org/1g5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g5c RCSB], [https://www.ebi.ac.uk/pdbsum/1g5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5c ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q50565_METTH Q50565_METTH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g5c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5c ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1G5C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus] with ZN, CA and EPE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G5C OCA].
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*[[Carbonic anhydrase 3D structures|Carbonic anhydrase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the "cab"-type beta class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicum., Strop P, Smith KS, Iverson TM, Ferry JG, Rees DC, J Biol Chem. 2001 Mar 30;276(13):10299-305. Epub 2000 Nov 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11096105 11096105]
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[[Category: Large Structures]]
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[[Category: Carbonate dehydratase]]
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[[Category: Methanothermobacter thermautotrophicus]]
[[Category: Methanothermobacter thermautotrophicus]]
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[[Category: Single protein]]
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[[Category: Ferry JG]]
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[[Category: Ferry, J.G.]]
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[[Category: Iverson TM]]
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[[Category: Iverson, T.M.]]
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[[Category: Rees DC]]
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[[Category: Rees, D.C.]]
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[[Category: Smith KS]]
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[[Category: Smith, K.S.]]
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[[Category: Strop P]]
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[[Category: Strop, P.]]
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[[Category: CA]]
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[[Category: EPE]]
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[[Category: ZN]]
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[[Category: beta carbonic anhydrase]]
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[[Category: hepes]]
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[[Category: zinc]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:51:32 2007''
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Current revision

CRYSTAL STRUCTURE OF THE 'CAB' TYPE BETA CLASS CARBONIC ANHYDRASE FROM METHANOBACTERIUM THERMOAUTOTROPHICUM

PDB ID 1g5c

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