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1g60

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[[Image:1g60.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of Methyltransferase MboIIa (Moraxella bovis)==
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|PDB= 1g60 |SIZE=350|CAPTION= <scene name='initialview01'>1g60</scene>, resolution 1.74&Aring;
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<StructureSection load='1g60' size='340' side='right'caption='[[1g60]], [[Resolution|resolution]] 1.74&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>
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<table><tr><td colspan='2'>[[1g60]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moraxella_bovis Moraxella bovis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G60 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.74&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g60 OCA], [https://pdbe.org/1g60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g60 RCSB], [https://www.ebi.ac.uk/pdbsum/1g60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g60 ProSAT], [https://www.topsan.org/Proteins/MCSG/1g60 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MTM2_MORBO MTM2_MORBO] This methylase recognizes the double-stranded sequence GAAGA, causes specific methylation on A-5, and protects the DNA from cleavage by the MboII endonuclease. It is not known if the cytosine of the complementary sequence TCTTC is also methylated by this enzyme.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g6/1g60_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g60 ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of Methyltransferase MboIIa (Moraxella bovis)'''
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==See Also==
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*[[DNA methyltransferase 3D structures|DNA methyltransferase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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DNA methyltransferases (MTases) are sequence-specific enzymes which transfer a methyl group from S-adenosyl-L-methionine (AdoMet) to the amino group of either cytosine or adenine within a recognized DNA sequence. Methylation of a base in a specific DNA sequence protects DNA from nucleolytic cleavage by restriction enzymes recognizing the same DNA sequence. We have determined at 1.74 A resolution the crystal structure of a beta-class DNA MTase MboIIA (M.MboIIA) from the bacterium Moraxella bovis, the smallest DNA MTase determined to date. M.MboIIA methylates the 3' adenine of the pentanucleotide sequence 5'-GAAGA-3'. The protein crystallizes with two molecules in the asymmetric unit which we propose to resemble the dimer when M.MboIIA is not bound to DNA. The overall structure of the enzyme closely resembles that of M.RsrI. However, the cofactor-binding pocket in M.MboIIA forms a closed structure which is in contrast to the open-form structures of other known MTases.
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[[Category: Large Structures]]
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==About this Structure==
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1G60 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Moraxella_bovis Moraxella bovis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G60 OCA].
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==Reference==
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Crystal structure of MboIIA methyltransferase., Osipiuk J, Walsh MA, Joachimiak A, Nucleic Acids Res. 2003 Sep 15;31(18):5440-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12954781 12954781]
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[[Category: Moraxella bovis]]
[[Category: Moraxella bovis]]
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[[Category: Single protein]]
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[[Category: Joachimiak A]]
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[[Category: Site-specific DNA-methyltransferase (adenine-specific)]]
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[[Category: Osipiuk J]]
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[[Category: Joachimiak, A.]]
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[[Category: Walsh MA]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Osipiuk, J.]]
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[[Category: Walsh, M A.]]
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[[Category: NA]]
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[[Category: SAM]]
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[[Category: dna methylation]]
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[[Category: mcsg]]
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[[Category: midwest center for structural genomic]]
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[[Category: moraxella bovi]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: s-adenosylmethionine]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:18:39 2008''
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Current revision

Crystal Structure of Methyltransferase MboIIa (Moraxella bovis)

PDB ID 1g60

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