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1g8y

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CRYSTAL STRUCTURE OF THE HEXAMERIC REPLICATIVE HELICASE REPA OF PLASMID RSF1010

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Retrieved from "http://52.214.119.220/wiki/index.php/1g8y"

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-[[Image:1g8y.gif|left|200px]]<br /><applet load="1g8y" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1g8y, resolution 2.40&Aring;" />
-'''CRYSTAL STRUCTURE OF THE HEXAMERIC REPLICATIVE HELICASE REPA OF PLASMID RSF1010'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF THE HEXAMERIC REPLICATIVE HELICASE REPA OF PLASMID RSF1010==
-Unwinding of double-stranded DNA into single-stranded intermediates, required for various fundamental life processes is catalyzed by helicases, a family of mono-, di- or hexameric motor proteins fueled by nucleoside, triphosphate hydrolysis. The three-dimensional crystal structure of the, hexameric helicase RepA encoded by plasmid RSF1010 has been determined by, X-ray diffraction at 2.4 A resolution. The hexamer shows an annular, structure with 6-fold rotational symmetry and a approximately 17 A wide, central hole, suggesting that single-stranded DNA may be threaded during, unwinding. Homologs of all five conserved sequence motifs of the DnaB-like, helicase family are found in RepA, and the topography of the monomer, resembles RecA and the helicase domain of the bacteriophage T7 gp4, protein. In a modeled complex, ATP molecules are located at the subunit, interfaces and clearly define adenine-binding and ATPase catalytic sites, formed by amino acid residues located on adjacent monomers; most, remarkable is the "arginine finger" Arg207 contributing to the active site, in the adjacent monomer. This arrangement of active-site residues suggests, cooperativity between monomers in ATP hydrolysis and helicase activity of, RepA. The mechanism of DNA unwinding remains elusive, as RepA is 6-fold, symmetric, contrasting the recently published asymmetric structure of the, bacteriophage T7 gp4 helicase domain.
+<StructureSection load='1g8y' size='340' side='right'caption='[[1g8y]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1g8y]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G8Y FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g8y OCA], [https://pdbe.org/1g8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g8y RCSB], [https://www.ebi.ac.uk/pdbsum/1g8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g8y ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/REPJ_ECOLX REPJ_ECOLX] This protein is involved in regulating the plasmid copy-number. Increasing the level of this protein results in a higher plasmid copy-number.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g8/1g8y_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g8y ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-G8Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G8Y OCA].
+*[[Helicase 3D structures|Helicase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Crystal structure of the hexameric replicative helicase RepA of plasmid RSF1010., Niedenzu T, Roleke D, Bains G, Scherzinger E, Saenger W, J Mol Biol. 2001 Feb 23;306(3):479-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11178907 11178907]
 [[Category: Escherichia coli]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Bains, G.]]
+[[Category: Bains G]]
-[[Category: Niedenzu, T.]]
+[[Category: Niedenzu T]]
-[[Category: Roeleke, D.]]
+[[Category: Roeleke D]]
-[[Category: Saenger, W.]]
+[[Category: Saenger W]]
-[[Category: Scherzinger, E.]]
+[[Category: Scherzinger E]]
-[[Category: p-loop]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:49:49 2007''

1g8y

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CRYSTAL STRUCTURE OF THE HEXAMERIC REPLICATIVE HELICASE REPA OF PLASMID RSF1010

Structural highlights

Function

Evolutionary Conservation

See Also

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