1g9q

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(New page: 200px<br /><applet load="1g9q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g9q, resolution 2.30&Aring;" /> '''COMPLEX STRUCTURE OF...)
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[[Image:1g9q.jpg|left|200px]]<br /><applet load="1g9q" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g9q, resolution 2.30&Aring;" />
 
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'''COMPLEX STRUCTURE OF THE ADPR-ASE AND ITS SUBSTRATE ADP-RIBOSE'''<br />
 
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==Overview==
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==COMPLEX STRUCTURE OF THE ADPR-ASE AND ITS SUBSTRATE ADP-RIBOSE==
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Regulation of cellular levels of ADP-ribose is important in preventing, nonenzymatic ADP-ribosylation of proteins. The Escherichia coli ADP-ribose, pyrophosphatase, a Nudix enzyme, catalyzes the hydrolysis of ADP-ribose to, ribose-5-P and AMP, compounds that can be recycled as part of nucleotide, metabolism. The structures of the apo enzyme, the active enzyme and the, complex with ADP-ribose were determined to 1.9 A, 2.7 A and 2.3 A, respectively. The structures reveal a symmetric homodimer with two, equivalent catalytic sites, each formed by residues of both monomers, requiring dimerization through domain swapping for substrate recognition, and catalytic activity. The structures also suggest a role for the, residues conserved in each Nudix subfamily. The Nudix motif residues, folded as a loop-helix-loop tailored for pyrophosphate hydrolysis, compose, the catalytic center; residues conferring substrate specificity occur in, regions of the sequence removed from the Nudix motif. This segregation of, catalytic and recognition roles provides versatility to the Nudix family.
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<StructureSection load='1g9q' size='340' side='right'caption='[[1g9q]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1g9q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G9Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G9Q FirstGlance]. <br>
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1G9Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with APR as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/ADP-ribose_diphosphatase ADP-ribose diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.13 3.6.1.13] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G9Q OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g9q OCA], [https://pdbe.org/1g9q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g9q RCSB], [https://www.ebi.ac.uk/pdbsum/1g9q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g9q ProSAT]</span></td></tr>
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The structure of ADP-ribose pyrophosphatase reveals the structural basis for the versatility of the Nudix family., Gabelli SB, Bianchet MA, Bessman MJ, Amzel LM, Nat Struct Biol. 2001 May;8(5):467-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11323725 11323725]
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</table>
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[[Category: ADP-ribose diphosphatase]]
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== Function ==
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[https://www.uniprot.org/uniprot/ADPP_ECOLI ADPP_ECOLI] Acts on ADP-mannose and ADP-glucose as well as ADP-ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process.<ref>PMID:11416161</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g9/1g9q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g9q ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Amzel, L.M.]]
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[[Category: Amzel LM]]
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[[Category: Bessman, M.J.]]
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[[Category: Bessman MJ]]
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[[Category: Bianchet, M.A.]]
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[[Category: Bianchet MA]]
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[[Category: Gabelli, S.B]]
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[[Category: Gabelli SB]]
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[[Category: APR]]
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[[Category: nudix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:51:39 2007''
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Current revision

COMPLEX STRUCTURE OF THE ADPR-ASE AND ITS SUBSTRATE ADP-RIBOSE

PDB ID 1g9q

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