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1gcb

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(New page: 200px<br /><applet load="1gcb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gcb, resolution 2.2&Aring;" /> '''GAL6, YEAST BLEOMYCIN...)
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[[Image:1gcb.jpg|left|200px]]<br /><applet load="1gcb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gcb, resolution 2.2&Aring;" />
 
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'''GAL6, YEAST BLEOMYCIN HYDROLASE DNA-BINDING PROTEASE (THIOL)'''<br />
 
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==Overview==
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==GAL6, YEAST BLEOMYCIN HYDROLASE DNA-BINDING PROTEASE (THIOL)==
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Bleomycin hydrolase is a cysteine protease that hydrolyzes the anticancer, drug bleomycin. The homolog in yeast, Gal6, has recently been identified, and found to bind DNA and to act as a repressor in the Gal4 regulatory, system. The crystal structure of Gal6 at 2.2 A resolution reveals a, hexameric structure with a prominent central channel. The papain-like, active sites are situated within the central channel, in a manner, resembling the organization of active sites in the proteasome. The Gal6, channel is lined with 60 lysine residues from the six subunits, suggesting, a role in DNA binding. The carboxyl-terminal arm of Gal6 extends into the, active site cleft and may serve a regulatory function. Rather than each, residing in distinct, separable domains, the protease and DNA-binding, activities appear structurally intertwined in the hexamer, implying a, coupling of these two activities.
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<StructureSection load='1gcb' size='340' side='right'caption='[[1gcb]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1gcb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GCB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GCB FirstGlance]. <br>
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1GCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SO4, HG and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GCB OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gcb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gcb OCA], [https://pdbe.org/1gcb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gcb RCSB], [https://www.ebi.ac.uk/pdbsum/1gcb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gcb ProSAT]</span></td></tr>
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Crystal structure of a conserved protease that binds DNA: the bleomycin hydrolase, Gal6., Joshua-Tor L, Xu HE, Johnston SA, Rees DC, Science. 1995 Aug 18;269(5226):945-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7638617 7638617]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BLH1_YEAST BLH1_YEAST] The normal physiological role of the enzyme is unknown, but it is not essential for the viability of yeast cells. Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity. Acts as a repressor in the GAL4 regulatory system, but this does not require either the peptidase or nucleic acid-binding activities.<ref>PMID:12555812</ref> <ref>PMID:16769724</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gc/1gcb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gcb ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Johnston SA]]
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[[Category: Johnston, S.A.]]
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[[Category: Joshua-Tor L]]
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[[Category: Joshua-Tor, L.]]
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[[Category: Rees DC]]
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[[Category: Rees, D.C.]]
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[[Category: Xu HE]]
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[[Category: Xu, H.E.]]
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[[Category: GOL]]
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[[Category: HG]]
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[[Category: SO4]]
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[[Category: bleomycin hydrolase]]
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[[Category: cysteine protease]]
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[[Category: dna-binding]]
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[[Category: peptidase]]
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[[Category: regulatory factor]]
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[[Category: ring protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:56:06 2007''
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Current revision

GAL6, YEAST BLEOMYCIN HYDROLASE DNA-BINDING PROTEASE (THIOL)

PDB ID 1gcb

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