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1get

From Proteopedia

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[[Image:1get.gif|left|200px]]<br /><applet load="1get" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1get, resolution 2.00&Aring;" />
 
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'''ANATOMY OF AN ENGINEERED NAD-BINDING SITE'''<br />
 
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==Overview==
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==ANATOMY OF AN ENGINEERED NAD-BINDING SITE==
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The coenzyme specificity of Escherichia coli glutathione reductase was switched from NADP to NAD by modifying the environment of the 2'-phosphate binding site through a set of point mutations: A179G, A183G, V197E, R198M, K199F, H200D, and R204P (Scrutton NS, Berry A, Perham RN, 1990, Nature 343:38-43). In order to analyze the structural changes involved, we have determined 4 high-resolution crystal structures, i.e., the structures of the wild-type enzyme (1.86 A resolution, R-factor of 16.8%), of the wild-type enzyme ligated with NADP (2.0 A, 20.8%), of the NAD-dependent mutant (1.74 A, 16.8%), and of the NAD-dependent mutant ligated with NAD (2.2 A, 16.9%). A comparison of these structures reveals subtle differences that explain details of the specificity change. In particular, a peptide rotation occurs close to the adenosine ribose, with a concomitant change of the ribose pucker. The mutations cause a contraction of the local chain fold. Furthermore, the engineered NAD-binding site assumes a less rigid structure than the NADP site of the wild-type enzyme. A superposition of the ligated structures shows a displacement of NAD versus NADP such that the electron pathway from the nicotinamide ring to FAD is elongated, which may explain the lower catalytic efficiency of the mutant. Because the nicotinamide is as much as 15 A from the sites of the mutations, this observation reminds us that mutations may have important long-range consequences that are difficult to anticipate.
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<StructureSection load='1get' size='340' side='right'caption='[[1get]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1get]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GET FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1get FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1get OCA], [https://pdbe.org/1get PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1get RCSB], [https://www.ebi.ac.uk/pdbsum/1get PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1get ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GSHR_ECOLI GSHR_ECOLI] Maintains high levels of reduced glutathione in the cytosol.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ge/1get_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1get ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1GET is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=NAP:'>NAP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutathione-disulfide_reductase Glutathione-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.7 1.8.1.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GET OCA].
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*[[Glutathione Reductase|Glutathione Reductase]]
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__TOC__
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==Reference==
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</StructureSection>
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Anatomy of an engineered NAD-binding site., Mittl PR, Berry A, Scrutton NS, Perham RN, Schulz GE, Protein Sci. 1994 Sep;3(9):1504-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7833810 7833810]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Glutathione-disulfide reductase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Mittl PRE]]
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[[Category: Mittl, P R.E.]]
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[[Category: Schulz GE]]
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[[Category: Schulz, G E.]]
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[[Category: FAD]]
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[[Category: NAP]]
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[[Category: oxidoreductase(flavoenzyme)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:49:15 2008''
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Current revision

ANATOMY OF AN ENGINEERED NAD-BINDING SITE

PDB ID 1get

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