1goc

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(New page: 200px<br /><applet load="1goc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1goc, resolution 2.0&Aring;" /> '''COOPERATIVE STABILIZA...)
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[[Image:1goc.gif|left|200px]]<br /><applet load="1goc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1goc, resolution 2.0&Aring;" />
 
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'''COOPERATIVE STABILIZATION OF ESCHERICHIA COLI RIBONUCLEASE HI BY INSERTION OF GLY-80B AND GLY-77-> ALA SUBSTITUTION'''<br />
 
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==Overview==
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==COOPERATIVE STABILIZATION OF ESCHERICHIA COLI RIBONUCLEASE HI BY INSERTION OF GLY-80B AND GLY-77-> ALA SUBSTITUTION==
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The insertion of a Gly residue (designated as Gly-80b) between the C-cap, of the alpha II-helix (Gln-80) and the N-cap of the alpha III-helix, (Trp-81) in Escherichia coli ribonuclease HI enhances the protein, stability by 0.4 kcal/mol in delta G (Kimura, S., Nakamura, H., Hashimoto, T., Oobatake, M., &amp; Kanaya, S. (1992) J. Biol. Chem. 267, 21535-21542)., Another mutation within the alpha II-helix, Gly-77--&gt;Ala, reduces the, stability by 0.9 kcal/mol. Simultaneous introduction of these mutations, enhances the stability by 0.8 kcal/mol, indicating that the effects of, these mutations are cooperative and not simply independent. We determined, the crystal structures of these three mutant proteins (G80b-, A77-, and, A77/G80b-RNase H) to investigate this cooperative mechanism of the protein, stabilization. The structures revealed that the inserted Gly-80b assumes a, left-handed helical conformation in both the G80b- and the A77/G80b-RNase, H. This inserted glycine residue allows the formation of a "paperclip", which is a common motif at the C-termini of alpha-helices. Accompanying, the formation of the paperclip motif, two intrahelical hydrogen bonds are, formed between the backbone atoms (O78-N80b and O80b-N84). The, stabilization caused by the insertion of Gly-80b can be ascribed to the, formation of these hydrogen bonds. The Gly-77--&gt;Ala substitution, destabilizes the protein due to the deformed packing interactions in the, hydrophobic core around Ala-77 and the stress in the wedged indole ring of, Trp-81. These effects are alleviated by the insertion of Gly-80b, which, relaxes the backbone structure.(ABSTRACT TRUNCATED AT 250 WORDS)
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<StructureSection load='1goc' size='340' side='right'caption='[[1goc]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1goc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GOC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1goc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1goc OCA], [https://pdbe.org/1goc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1goc RCSB], [https://www.ebi.ac.uk/pdbsum/1goc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1goc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNH_ECOLI RNH_ECOLI] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer.[HAMAP-Rule:MF_00042]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/1goc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1goc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1GOC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GOC OCA].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Cooperative stabilization of Escherichia coli ribonuclease HI by insertion of Gly-80b and Gly-77--&gt;Ala substitution., Ishikawa K, Nakamura H, Morikawa K, Kimura S, Kanaya S, Biochemistry. 1993 Jul 20;32(28):7136-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8393706 8393706]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Ribonuclease H]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Ishikawa K]]
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[[Category: Ishikawa, K.]]
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[[Category: Kanaya S]]
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[[Category: Kanaya, S.]]
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[[Category: Kimura S]]
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[[Category: Kimura, S.]]
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[[Category: Morikawa K]]
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[[Category: Morikawa, K.]]
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[[Category: Nakamura H]]
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[[Category: Nakamura, H.]]
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[[Category: hydrolase(endoribonuclease)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:12:04 2007''
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Current revision

COOPERATIVE STABILIZATION OF ESCHERICHIA COLI RIBONUCLEASE HI BY INSERTION OF GLY-80B AND GLY-77-> ALA SUBSTITUTION

PDB ID 1goc

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