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1grz

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[[Image:1grz.gif|left|200px]]<br /><applet load="1grz" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1grz, resolution 5.0&Aring;" />
 
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'''A PREORGANIZED ACTIVE SITE IN THE CRYSTAL STRUCTURE OF THE TETRAHYMENA RIBOZYME'''<br />
 
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==Overview==
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==A PREORGANIZED ACTIVE SITE IN THE CRYSTAL STRUCTURE OF THE TETRAHYMENA RIBOZYME==
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Group I introns possess a single active site that catalyzes the two sequential reactions of self-splicing. An RNA comprising the two domains of the Tetrahymena thermophila group I intron catalytic core retains activity, and the 5.0 angstrom crystal structure of this 247-nucleotide ribozyme is now described. Close packing of the two domains forms a shallow cleft capable of binding the short helix that contains the 5' splice site. The helix that provides the binding site for the guanosine substrate deviates significantly from A-form geometry, providing a tight binding pocket. The binding pockets for both the 5' splice site helix and guanosine are formed and oriented in the absence of these substrates. Thus, this large ribozyme is largely preorganized for catalysis, much like a globular protein enzyme.
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<StructureSection load='1grz' size='340' side='right'caption='[[1grz]], [[Resolution|resolution]] 5.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1grz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GRZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1grz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1grz OCA], [https://pdbe.org/1grz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1grz RCSB], [https://www.ebi.ac.uk/pdbsum/1grz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1grz ProSAT]</span></td></tr>
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</table>
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==About this Structure==
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==See Also==
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1GRZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GRZ OCA].
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*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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==Reference==
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__TOC__
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A preorganized active site in the crystal structure of the Tetrahymena ribozyme., Golden BL, Gooding AR, Podell ER, Cech TR, Science. 1998 Oct 9;282(5387):259-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9841391 9841391]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
[[Category: Tetrahymena thermophila]]
[[Category: Tetrahymena thermophila]]
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[[Category: Cech, T R.]]
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[[Category: Cech TR]]
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[[Category: Golden, B L.]]
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[[Category: Golden BL]]
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[[Category: Gooding, A R.]]
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[[Category: Gooding AR]]
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[[Category: Podell, E R.]]
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[[Category: Podell ER]]
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[[Category: ribozyme structure]]
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[[Category: rrna group i intron]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:53:21 2008''
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Current revision

A PREORGANIZED ACTIVE SITE IN THE CRYSTAL STRUCTURE OF THE TETRAHYMENA RIBOZYME

PDB ID 1grz

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