1gwz

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[[Image:1gwz.jpg|left|200px]]<br /><applet load="1gwz" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gwz, resolution 2.50&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE PROTEIN TYROSINE PHOSPHATASE SHP-1'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE PROTEIN TYROSINE PHOSPHATASE SHP-1==
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The crystal structures of the protein-tyrosine phosphatase SHP-1 catalytic, domain and the complex it forms with the substrate analogue tungstate have, been determined and refined to crystallographic R values of 0.209 at 2.5 A, resolution and 0.207 at 2.8 A resolution, respectively. Despite low, sequence similarity, the catalytic domain of SHP-1 shows high similarity, in secondary and tertiary structures with other protein-tyrosine, phosphatases (PTPs). In contrast to the conformational changes observed in, the crystal structures of PTP1B and Yersinia PTP, the WPD loop, (Trp419-Pro428) in the catalytic domain of SHP-1 moves away from the, substrate binding pocket after binding the tungstate ion. Sequence, alignment and structural analysis suggest that the residues in the WPD, loop, especially the amino acid following Asp421, are critical for the, movement of WPD loop on binding substrates and the specific activity of, protein-tyrosine phosphatases. Our mutagenesis and kinetic measurements, have supported this hypothesis.
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<StructureSection load='1gwz' size='340' side='right'caption='[[1gwz]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gwz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GWZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gwz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gwz OCA], [https://pdbe.org/1gwz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gwz RCSB], [https://www.ebi.ac.uk/pdbsum/1gwz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gwz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PTN6_HUMAN PTN6_HUMAN] Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.<ref>PMID:11266449</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gw/1gwz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gwz ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1GWZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] Known structural/functional Site: <scene name='pdbsite=WPD:Involved+In+Substrate+Binding'>WPD</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWZ OCA].
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*[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of the catalytic domain of protein-tyrosine phosphatase SHP-1., Yang J, Liang X, Niu T, Meng W, Zhao Z, Zhou GW, J Biol Chem. 1998 Oct 23;273(43):28199-207. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9774441 9774441]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein-tyrosine-phosphatase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Liang X]]
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[[Category: Liang, X.]]
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[[Category: Meng W]]
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[[Category: Meng, W.]]
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[[Category: Niu T]]
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[[Category: Niu, T.]]
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[[Category: Yang J]]
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[[Category: Yang, J.]]
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[[Category: Zhao Z]]
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[[Category: Zhao, Z.]]
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[[Category: Zhou GW]]
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[[Category: Zhou, G.W.]]
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[[Category: catalytic domain]]
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[[Category: hydrolase]]
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[[Category: protein tyrosine phosphatase]]
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[[Category: sh2 domain]]
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[[Category: wpd loop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:43:40 2008''
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Current revision

CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE PROTEIN TYROSINE PHOSPHATASE SHP-1

PDB ID 1gwz

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