1hjr

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(New page: 200px<br /><applet load="1hjr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hjr, resolution 2.5&Aring;" /> '''ATOMIC STRUCTURE OF T...)
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[[Image:1hjr.jpg|left|200px]]<br /><applet load="1hjr" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hjr, resolution 2.5&Aring;" />
 
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'''ATOMIC STRUCTURE OF THE RUVC RESOLVASE: A HOLLIDAY JUNCTION-SPECIFIC ENDONUCLEASE FROM E. COLI'''<br />
 
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==Overview==
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==ATOMIC STRUCTURE OF THE RUVC RESOLVASE: A HOLLIDAY JUNCTION-SPECIFIC ENDONUCLEASE FROM E. COLI==
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The crystal structure of the RuvC protein, a Holliday junction resolvase, from E. coli, has been determined at 2.5 A resolution. The enzyme forms a, dimer of 19 kDa subunits related by a dyad axis. Together with results, from extensive mutational analyses, the refined structure reveals that the, catalytic center, comprising four acidic residues, lies at the bottom of a, cleft that nicely fits a DNA duplex. The structural features of the dimer, with a 30 A spacing between the two catalytic centers, provide a, substantially defined image of the Holliday junction architecture. The, folding topology in the vicinity of the catalytic site exhibits a striking, similarity to that of RNAase H1 from E. coli.
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<StructureSection load='1hjr' size='340' side='right'caption='[[1hjr]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1hjr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HJR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjr OCA], [https://pdbe.org/1hjr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hjr RCSB], [https://www.ebi.ac.uk/pdbsum/1hjr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hjr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RUVC_ECOLI RUVC_ECOLI] Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.<ref>PMID:1661673</ref> <ref>PMID:1758493</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/1hjr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hjr ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1HJR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HJR OCA].
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*[[Resolvase 3D structures|Resolvase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli., Ariyoshi M, Vassylyev DG, Iwasaki H, Nakamura H, Shinagawa H, Morikawa K, Cell. 1994 Sep 23;78(6):1063-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7923356 7923356]
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__TOC__
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[[Category: Crossover junction endodeoxyribonuclease]]
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ariyoshi, M.]]
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[[Category: Ariyoshi M]]
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[[Category: Morikawa, K.]]
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[[Category: Morikawa K]]
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[[Category: Vassylyev, D.G.]]
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[[Category: Vassylyev DG]]
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[[Category: site-specific recombinase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:36:52 2007''
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Current revision

ATOMIC STRUCTURE OF THE RUVC RESOLVASE: A HOLLIDAY JUNCTION-SPECIFIC ENDONUCLEASE FROM E. COLI

PDB ID 1hjr

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