This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1hrm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:29, 7 February 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1hrm.jpg|left|200px]]
 
-
<!--
+
==THE PROXIMAL LIGAND VARIANT HIS93TYR OF HORSE HEART MYOGLOBIN==
-
The line below this paragraph, containing "STRUCTURE_1hrm", creates the "Structure Box" on the page.
+
<StructureSection load='1hrm' size='340' side='right'caption='[[1hrm]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1hrm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HRM FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
{{STRUCTURE_1hrm| PDB=1hrm | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hrm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hrm OCA], [https://pdbe.org/1hrm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hrm RCSB], [https://www.ebi.ac.uk/pdbsum/1hrm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hrm ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MYG_HORSE MYG_HORSE] Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hr/1hrm_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hrm ConSurf].
 +
<div style="clear:both"></div>
-
'''THE PROXIMAL LIGAND VARIANT HIS93TYR OF HORSE HEART MYOGLOBIN'''
+
==See Also==
-
 
+
*[[Myoglobin 3D structures|Myoglobin 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The spectroscopic and structural properties of the His93Tyr variant of horse heart myoglobin have been studied to assess the effects of replacing the proximal His residue of this protein with a tyrosyl residue as occurs in catalases from various sources. The variant in the ferric form exhibits electronic spectra that are independent of pH between pH 7 and 10, and it exhibits changes in absorption maxima and intensity that are consistent with a five-coordinate heme iron center at the active site. The EPR spectrum of the variant is that of a high-spin, rhombic system similar to that reported for bovine liver catalase. The 1D 1H-NMR spectrum of the variant confirms the five-coordinate nature of the heme iron center and exhibits a broad resonance at 112.5 ppm that is attributable to the meta protons of the phenolate ligand. This result indicates that the new Tyr ligand flips at a significant rate in this protein. The thermal stability of the Fe(III) derivative is unchanged from that of the wild-type protein (pH 8) while the midpoint reduction potential [-208 mV vs SHE (pH 8.0, 25 degrees C)] is about 250 mV lower. The three-dimensional structure of the variant determined by X-ray diffraction analysis confirms the five-coordinate nature of the heme iron center and establishes that the introduction of a proximal Tyr ligand is accommodated by a shift of the F helix (residues 88-99) in which this residue resides away from the heme pocket. Additional effects of this change are small shifts in the positions of Leu29, a heme propionate, and a heme vinyl group that are accompanied by altered hydrogen bonding interactions with the heme prosthetic group.(ABSTRACT TRUNCATED AT 250 WORDS)
+
-
 
+
-
==About this Structure==
+
-
1HRM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HRM OCA].
+
-
 
+
-
==Reference==
+
-
The proximal ligand variant His93Tyr of horse heart myoglobin., Hildebrand DP, Burk DL, Maurus R, Ferrer JC, Brayer GD, Mauk AG, Biochemistry. 1995 Feb 14;34(6):1997-2005. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7849057 7849057]
+
[[Category: Equus caballus]]
[[Category: Equus caballus]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Brayer, G D.]]
+
[[Category: Brayer GD]]
-
[[Category: Burk, D L.]]
+
[[Category: Burk DL]]
-
[[Category: Oxygen transport]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:09:53 2008''
+

Current revision

THE PROXIMAL LIGAND VARIANT HIS93TYR OF HORSE HEART MYOGLOBIN

PDB ID 1hrm

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1hrm"
Personal tools