1i1q

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[[Image:1i1q.gif|left|200px]]
 
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{{Structure
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==STRUCTURE OF THE COOPERATIVE ALLOSTERIC ANTHRANILATE SYNTHASE FROM SALMONELLA TYPHIMURIUM==
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|PDB= 1i1q |SIZE=350|CAPTION= <scene name='initialview01'>1i1q</scene>, resolution 1.90&Aring;
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<StructureSection load='1i1q' size='340' side='right'caption='[[1i1q]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene>
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<table><tr><td colspan='2'>[[1i1q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I1Q FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i1q OCA], [https://pdbe.org/1i1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i1q RCSB], [https://www.ebi.ac.uk/pdbsum/1i1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i1q ProSAT]</span></td></tr>
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</table>
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'''STRUCTURE OF THE COOPERATIVE ALLOSTERIC ANTHRANILATE SYNTHASE FROM SALMONELLA TYPHIMURIUM'''
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== Function ==
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[https://www.uniprot.org/uniprot/TRPE_SALTY TRPE_SALTY] Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.<ref>PMID:4358945</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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We have determined the X-ray crystal structure of the cooperative anthranilate synthase heterotetramer from Salmonella typhimurium at 1.9 A resolution with the allosteric inhibitor l-tryptophan bound to a regulatory site in the TrpE subunit. Tryptophan binding orders a loop that in turn stabilizes the inactive T state of the enzyme by restricting closure of the active site cleft. Comparison with the structure of the unliganded, noncooperative anthranilate synthase heterotetramer from Sulfolobus solfataricus shows that the two homologs have completely different quarternary structures, even though their functional dimer pairs are structurally similar, consistent with differences in the cooperative behavior of the enzymes. The structural model rationalizes mutational and biochemical studies of the enzyme and establishes the structural differences between cooperative and noncooperative anthranilate synthase homologs.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i1/1i1q_consurf.spt"</scriptWhenChecked>
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1I1Q is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I1Q OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of the cooperative allosteric anthranilate synthase from Salmonella typhimurium., Morollo AA, Eck MJ, Nat Struct Biol. 2001 Mar;8(3):243-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11224570 11224570]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i1q ConSurf].
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[[Category: Anthranilate synthase]]
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<div style="clear:both"></div>
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[[Category: Protein complex]]
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== References ==
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[[Category: Salmonella typhimurium]]
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<references/>
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[[Category: Eck, M J.]]
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__TOC__
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[[Category: Morollo, A A.]]
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</StructureSection>
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[[Category: TRP]]
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[[Category: Large Structures]]
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[[Category: tryptophan biosynthesis]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Eck MJ]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:44:23 2008''
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[[Category: Morollo AA]]

Current revision

STRUCTURE OF THE COOPERATIVE ALLOSTERIC ANTHRANILATE SYNTHASE FROM SALMONELLA TYPHIMURIUM

PDB ID 1i1q

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