This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1i52

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1i52" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i52, resolution 1.50&Aring;" /> '''CRYSTAL STRUCTURE OF...)
Current revision (07:32, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1i52.gif|left|200px]]<br /><applet load="1i52" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1i52, resolution 1.50&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF 4-DIPHOSPHOCYTIDYL-2-C-METHYLERYTHRITOL (CDP-ME) SYNTHASE (YGBP) INVOLVED IN MEVALONATE INDEPENDENT ISOPRENOID BIOSYNTHESIS'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF 4-DIPHOSPHOCYTIDYL-2-C-METHYLERYTHRITOL (CDP-ME) SYNTHASE (YGBP) INVOLVED IN MEVALONATE INDEPENDENT ISOPRENOID BIOSYNTHESIS==
-
The YgbP protein of Escherichia coli encodes the enzyme, 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) synthetase, a member of, the cytidyltransferase family of enzymes. CDP-ME is an intermediate in the, mevalonate-independent pathway for isoprenoid biosynthesis in a number of, prokaryotic organisms, algae, the plant plastids and the malaria parasite., Because vertebrates synthesize isoprenoid precursors using a mevalonate, pathway, CDP-ME synthetase and other enzymes of the mevalonate-independent, pathway for isoprenoid production represent attractive targets for the, structure-based design of selective antibacterial, herbicidal and, antimalarial drugs. The high-resolution structures of E. coli CDP-ME, synthetase in the apo form and complexed with both CTP-Mg2+ and, CDP-ME-Mg2+ reveal the stereochemical principles underlying both substrate, and product recognition as well as catalysis in CDP-ME synthetase., Moreover, these complexes represent the first experimental structures for, any cytidyltransferase with both substrates and products bound.
+
<StructureSection load='1i52' size='340' side='right'caption='[[1i52]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1i52]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I52 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I52 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CTP:CYTIDINE-5-TRIPHOSPHATE'>CTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i52 OCA], [https://pdbe.org/1i52 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i52 RCSB], [https://www.ebi.ac.uk/pdbsum/1i52 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i52 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ISPD_ECOLI ISPD_ECOLI] Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).[HAMAP-Rule:MF_00108]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/1i52_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i52 ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1I52 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CA, MG and CTP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I52 OCA].
+
*[[MEP cytidylyltransferase|MEP cytidylyltransferase]]
-
 
+
*[[MEP cytidylyltransferase 3D structures|MEP cytidylyltransferase 3D structures]]
-
==Reference==
+
__TOC__
-
Structure of 4-diphosphocytidyl-2-C- methylerythritol synthetase involved in mevalonate- independent isoprenoid biosynthesis., Richard SB, Bowman ME, Kwiatkowski W, Kang I, Chow C, Lillo AM, Cane DE, Noel JP, Nat Struct Biol. 2001 Jul;8(7):641-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11427897 11427897]
+
</StructureSection>
-
[[Category: Escherichia coli]]
+
[[Category: Escherichia coli K-12]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Bowman, M.E.]]
+
[[Category: Bowman ME]]
-
[[Category: Cane, D.E.]]
+
[[Category: Cane DE]]
-
[[Category: Chow, C.]]
+
[[Category: Chow C]]
-
[[Category: Kang, I.]]
+
[[Category: Kang I]]
-
[[Category: Kwiatkowski, W.]]
+
[[Category: Kwiatkowski W]]
-
[[Category: Lillo, M.]]
+
[[Category: Lillo M]]
-
[[Category: Noel, J.P.]]
+
[[Category: Noel JP]]
-
[[Category: Richard, S.B.]]
+
[[Category: Richard SB]]
-
[[Category: CA]]
+
-
[[Category: CTP]]
+
-
[[Category: MG]]
+
-
[[Category: cytidylyltransferase]]
+
-
[[Category: deoxyxylulose-5-phosphate pathway (dxp)]]
+
-
[[Category: isoprenoid biosynthesys]]
+
-
[[Category: mep]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:03:33 2007''
+

Current revision

CRYSTAL STRUCTURE OF 4-DIPHOSPHOCYTIDYL-2-C-METHYLERYTHRITOL (CDP-ME) SYNTHASE (YGBP) INVOLVED IN MEVALONATE INDEPENDENT ISOPRENOID BIOSYNTHESIS

PDB ID 1i52

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1i52"
Personal tools