This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1i6i

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1i6i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i6i, resolution 2.00&Aring;" /> '''CRYSTAL STRUCTURE OF...)
Current revision (07:32, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1i6i.jpg|left|200px]]<br /><applet load="1i6i" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1i6i, resolution 2.00&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF THE KIF1A MOTOR DOMAIN COMPLEXED WITH MG-AMPPCP'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF THE KIF1A MOTOR DOMAIN COMPLEXED WITH MG-AMPPCP==
-
Kinesin motors are specialized enzymes that use hydrolysis of ATP to, generate force and movement along their cellular tracks, the microtubules., Although numerous biochemical and biophysical studies have accumulated, much data that link microtubule-assisted ATP hydrolysis to kinesin motion, the structural view of kinesin movement remains unclear. This study of the, monomeric kinesin motor KIF1A combines X-ray crystallography and, cryo-electron microscopy, and allows analysis of force-generating, conformational changes at atomic resolution. The motor is revealed in its, two functionally critical states-complexed with ADP and with a, non-hydrolysable analogue of ATP. The conformational change observed, between the ADP-bound and the ATP-like structures of the KIF1A catalytic, core is modular, extends to all kinesins and is similar to the, conformational change used by myosin motors and G proteins. Docking of the, ADP-bound and ATP-like crystallographic models of KIF1A into the, corresponding cryo-electron microscopy maps suggests a rationale for the, plus-end directional bias associated with the kinesin catalytic core.
+
<StructureSection load='1i6i' size='340' side='right'caption='[[1i6i]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1i6i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I6I FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i6i OCA], [https://pdbe.org/1i6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i6i RCSB], [https://www.ebi.ac.uk/pdbsum/1i6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i6i ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/KIF1A_MOUSE KIF1A_MOUSE] Motor for anterograde axonal transport of synaptic vesicle precursors.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i6/1i6i_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i6i ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1I6I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MG, ACP and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I6I OCA].
+
*[[Kinesin 3D Structures|Kinesin 3D Structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Switch-based mechanism of kinesin motors., Kikkawa M, Sablin EP, Okada Y, Yajima H, Fletterick RJ, Hirokawa N, Nature. 2001 May 24;411(6836):439-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11373668 11373668]
+
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Single protein]]
+
[[Category: Fletterick RJ]]
-
[[Category: Fletterick, R.J.]]
+
[[Category: Hirokawa N]]
-
[[Category: Hirokawa, N.]]
+
[[Category: Kikkawa M]]
-
[[Category: Kikkawa, M.]]
+
[[Category: Okada Y]]
-
[[Category: Okada, Y.]]
+
[[Category: Sablin EP]]
-
[[Category: Sablin, E.P.]]
+
[[Category: Yajima H]]
-
[[Category: Yajima, H.]]
+
-
[[Category: ACP]]
+
-
[[Category: MG]]
+
-
[[Category: TRS]]
+
-
[[Category: catalytic core]]
+
-
[[Category: kinesin]]
+
-
[[Category: motor protein]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:05:43 2007''
+

Current revision

CRYSTAL STRUCTURE OF THE KIF1A MOTOR DOMAIN COMPLEXED WITH MG-AMPPCP

PDB ID 1i6i

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1i6i"
Personal tools