1i8t

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[[Image:1i8t.jpg|left|200px]]<br /><applet load="1i8t" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1i8t, resolution 2.4&Aring;" />
 
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'''STRCUTURE OF UDP-GALACTOPYRANOSE MUTASE FROM E.COLI'''<br />
 
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==Overview==
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==STRUCTURE OF UDP-GALACTOPYRANOSE MUTASE FROM E.COLI==
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Uridine diphosphogalactofuranose (UDP-Galf ) is the precursor of the d-galactofuranose (Galf ) residues found in bacterial and parasitic cell walls, including those of many pathogens, such as Mycobacterium tuberculosis and Trypanosoma cruzi. UDP-Galf is made from UDP-galactopyranose (UDP-Galp) by the enzyme UDP-galactopyranose mutase (mutase). The mutase enzyme is essential for the viability of mycobacteria and is not found in humans, making it a viable therapeutic target. The mechanism by which mutase achieves the unprecedented ring contraction of a nonreducing sugar is unclear. We have solved the crystal structure of Escherichia coli mutase to 2.4 A resolution. The novel structure shows that the flavin nucleotide is located in a cleft lined with conserved residues. Site-directed mutagenesis studies indicate that this cleft contains the active site, with the sugar ring of the substrate UDP-galactose adjacent to the exposed isoalloxazine ring of FAD. Assay results establish that the enzyme is active only when flavin is reduced. We conclude that mutase most likely functions by transient reduction of substrate.
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<StructureSection load='1i8t' size='340' side='right'caption='[[1i8t]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1i8t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I8T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8t OCA], [https://pdbe.org/1i8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i8t RCSB], [https://www.ebi.ac.uk/pdbsum/1i8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLF_ECOLI GLF_ECOLI] Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF) (By similarity).<ref>PMID:8576037</ref> <ref>PMID:11448178</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/1i8t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i8t ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1I8T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8T OCA].
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*[[UDP-galactopyranose mutase 3D structures|UDP-galactopyranose mutase 3D structures]]
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== References ==
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==Reference==
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<references/>
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UDP-galactopyranose mutase has a novel structure and mechanism., Sanders DA, Staines AG, McMahon SA, McNeil MR, Whitfield C, Naismith JH, Nat Struct Biol. 2001 Oct;8(10):858-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11573090 11573090]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: UDP-galactopyranose mutase]]
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[[Category: McMahon SA]]
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[[Category: McMahon, S A.]]
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[[Category: McNeil MR]]
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[[Category: McNeil, M R.]]
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[[Category: Naismith JH]]
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[[Category: Naismith, J H.]]
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[[Category: Sanders DAR]]
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[[Category: Sanders, D A.R.]]
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[[Category: Staines AG]]
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[[Category: Staines, A G.]]
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[[Category: Whitfield C]]
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[[Category: Whitfield, C.]]
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[[Category: FAD]]
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[[Category: contractase]]
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[[Category: fad]]
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[[Category: mutase]]
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[[Category: rossman fold]]
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[[Category: udp-galactopyranose]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:12 2008''
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Current revision

STRUCTURE OF UDP-GALACTOPYRANOSE MUTASE FROM E.COLI

PDB ID 1i8t

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