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1iap

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[[Image:1iap.jpg|left|200px]]<br /><applet load="1iap" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1iap, resolution 1.90&Aring;" />
 
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'''CRYSTAL STRUCTURE OF P115RHOGEF RGRGS DOMAIN'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF P115RHOGEF RGRGS DOMAIN==
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p115RhoGEF, a guanine nucleotide exchange factor for Rho GTPase, is also a, GTPase activating protein (GAP) for G(12) and G(13) heterotrimeric G alpha, subunits. Near its N-terminus, p115RhoGEF contains a domain (rgRGS) with, remote sequence identity to RGS (regulators of G protein signaling), domains. The rgRGS domain is necessary but not sufficient for the GAP, activity of p115RhoGEF. The 1.9 A resolution crystal structure of the, rgRGS domain shows structural similarity to RGS domains but possesses a, C-terminal extension that folds into a layer of helices that pack against, the hydrophobic core of the domain. Mutagenesis experiments show that, rgRGS may form interactions with G alpha(13) that are analogous to those, in complexes of RGS proteins with their G alpha substrates.
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<StructureSection load='1iap' size='340' side='right'caption='[[1iap]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1iap]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IAP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IAP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iap OCA], [https://pdbe.org/1iap PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iap RCSB], [https://www.ebi.ac.uk/pdbsum/1iap PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iap ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARHG1_HUMAN ARHG1_HUMAN] Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.<ref>PMID:8810315</ref> <ref>PMID:9641915</ref> <ref>PMID:9641916</ref> <ref>PMID:20098430</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/1iap_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1iap ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known disease associated with this structure: Slowed nerve conduction velocity, AD OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=608136 608136]]
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*[[Rho guanine nucleotide exchange factor 3D structures|Rho guanine nucleotide exchange factor 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1IAP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IAP OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Structure of the rgRGS domain of p115RhoGEF., Chen Z, Wells CD, Sternweis PC, Sprang SR, Nat Struct Biol. 2001 Sep;8(9):805-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11524686 11524686]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chen, Z.]]
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[[Category: Chen Z]]
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[[Category: Sprang, S.R.]]
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[[Category: Sprang SR]]
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[[Category: p115]]
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[[Category: rgrgs]]
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[[Category: rgs]]
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[[Category: rhogef]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:00:28 2008''
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Current revision

CRYSTAL STRUCTURE OF P115RHOGEF RGRGS DOMAIN

PDB ID 1iap

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