This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1ide

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1ide" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ide, resolution 2.5&Aring;" /> '''ISOCITRATE DEHYDROGEN...)
Current revision (07:34, 7 February 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ide.jpg|left|200px]]<br /><applet load="1ide" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ide, resolution 2.5&Aring;" />
 
-
'''ISOCITRATE DEHYDROGENASE Y160F MUTANT STEADY-STATE INTERMEDIATE COMPLEX (LAUE DETERMINATION)'''<br />
 
-
==Overview==
+
==ISOCITRATE DEHYDROGENASE Y160F MUTANT STEADY-STATE INTERMEDIATE COMPLEX (LAUE DETERMINATION)==
-
Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution, were used to solve the structures of two sequential intermediates formed, during the catalytic actions of isocitrate dehydrogenase. Both, intermediates are distinct from the enzyme-substrate and enzyme-product, complexes. Mutation of key catalytic residues changed the rate determining, steps so that protein and substrate intermediates within the overall, reaction pathway could be visualized.
+
<StructureSection load='1ide' size='340' side='right'caption='[[1ide]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ide]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The September 2010 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Isocitrate Dehydrogenase'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2010_9 10.2210/rcsb_pdb/mom_2010_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IDE FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ICT:ISOCITRIC+ACID'>ICT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ide FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ide OCA], [https://pdbe.org/1ide PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ide RCSB], [https://www.ebi.ac.uk/pdbsum/1ide PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ide ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/IDH_ECOLI IDH_ECOLI]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/id/1ide_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ide ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1IDE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG, ICT and NAP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase_(NADP(+)) Isocitrate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IDE OCA].
+
*[[Isocitrate dehydrogenase 3D structures|Isocitrate dehydrogenase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase., Bolduc JM, Dyer DH, Scott WG, Singer P, Sweet RM, Koshland DE Jr, Stoddard BL, Science. 1995 Jun 2;268(5215):1312-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7761851 7761851]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Isocitrate dehydrogenase (NADP(+))]]
+
[[Category: Isocitrate Dehydrogenase]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Bolduc, J.M.]]
+
[[Category: RCSB PDB Molecule of the Month]]
-
[[Category: Dyer, D.H.]]
+
[[Category: Bolduc JM]]
-
[[Category: Junior, D.E.Koshland.]]
+
[[Category: Dyer DH]]
-
[[Category: Scott, W.G.]]
+
[[Category: Koshland Junior DE]]
-
[[Category: Singer, P.]]
+
[[Category: Scott WG]]
-
[[Category: Stoddard, B.L.]]
+
[[Category: Singer P]]
-
[[Category: Sweet, R.M.]]
+
[[Category: Stoddard BL]]
-
[[Category: ICT]]
+
[[Category: Sweet RM]]
-
[[Category: MG]]
+
-
[[Category: NAP]]
+
-
[[Category: oxidoreductase (nad(a)-choh(d))]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:18:37 2007''
+

Current revision

ISOCITRATE DEHYDROGENASE Y160F MUTANT STEADY-STATE INTERMEDIATE COMPLEX (LAUE DETERMINATION)

PDB ID 1ide

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ide"
Personal tools