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1if0

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[[Image:1if0.gif|left|200px]]<br /><applet load="1if0" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1if0, resolution 12.&Aring;" />
 
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'''PSEUDO-ATOMIC MODEL OF BACTERIOPHAGE HK97 PROCAPSID (PROHEAD II)'''<br />
 
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==Overview==
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==PSEUDO-ATOMIC MODEL OF BACTERIOPHAGE HK97 PROCAPSID (PROHEAD II)==
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Large-scale conformational changes transform viral precursors into infectious virions. The structure of bacteriophage HK97 capsid, Head-II, was recently solved by crystallography, revealing a catenated cross-linked topology. We have visualized its precursor, Prohead-II, by cryoelectron microscopy and modeled the conformational change by appropriately adapting Head-II. Rigid-body rotations ( approximately 40 degrees) cause switching to an entirely different set of interactions; in addition, two motifs undergo refolding. These changes stabilize the capsid by increasing the surface area buried at interfaces and bringing the cross-link-forming residues, initially approximately 40 angstroms apart, close together. The inner surface of Prohead-II is negatively charged, suggesting that the transition is triggered electrostatically by DNA packaging.
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<SX load='1if0' size='340' side='right' viewer='molstar' caption='[[1if0]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1if0]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_HK97 Escherichia virus HK97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IF0 FirstGlance]. <br>
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1IF0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk620 Enterobacteria phage hk620]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IF0 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1if0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1if0 OCA], [https://pdbe.org/1if0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1if0 RCSB], [https://www.ebi.ac.uk/pdbsum/1if0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1if0 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Virus maturation involving large subunit rotations and local refolding., Conway JF, Wikoff WR, Cheng N, Duda RL, Hendrix RW, Johnson JE, Steven AC, Science. 2001 Apr 27;292(5517):744-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11326105 11326105]
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== Function ==
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[[Category: Enterobacteria phage hk620]]
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[https://www.uniprot.org/uniprot/CAPSD_BPHK7 CAPSD_BPHK7] Assembles to form an icosahedral capsid of 66 nm, with a T=7 laevo symmetry (PubMed:11000116, PubMed:21276801). Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function.<ref>PMID:11000116</ref> <ref>PMID:21276801</ref> <ref>PMID:7669350</ref> <ref>PMID:7723020</ref>
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Cheng, N.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Conway, J F.]]
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Check<jmol>
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[[Category: Duda, R L.]]
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<jmolCheckbox>
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[[Category: Hendrix, R W.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/if/1if0_consurf.spt"</scriptWhenChecked>
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[[Category: Johnson, J E.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Steven, A C.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Wikoff, W R.]]
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</jmolCheckbox>
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[[Category: bacteriophage]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1if0 ConSurf].
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[[Category: capsid]]
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<div style="clear:both"></div>
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[[Category: cryoem]]
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== References ==
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[[Category: icosahedral virus]]
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<references/>
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[[Category: pseudo-atomic model.]]
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__TOC__
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[[Category: virus]]
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</SX>
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[[Category: Escherichia virus HK97]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:13 2008''
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[[Category: Large Structures]]
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[[Category: Cheng N]]
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[[Category: Conway JF]]
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[[Category: Duda RL]]
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[[Category: Hendrix RW]]
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[[Category: Johnson JE]]
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[[Category: Steven AC]]
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[[Category: Wikoff WR]]

Current revision

PSEUDO-ATOMIC MODEL OF BACTERIOPHAGE HK97 PROCAPSID (PROHEAD II)

1if0, resolution 12.00Å

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OCA

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