This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1ig5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:35, 7 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ig5.gif|left|200px]]<br /><applet load="1ig5" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ig5, resolution 1.50&Aring;" />
 
-
'''BOVINE CALBINDIN D9K BINDING MG2+'''<br />
 
-
==Overview==
+
==BOVINE CALBINDIN D9K BINDING MG2+==
-
The three-dimensional structures of the magnesium- and manganese-bound, forms of calbindin D9k were determined to 1.6 A and 1.9 A resolution, respectively, using X-ray crystallography. These two structures are nearly, identical but deviate significantly from both the calcium bound form and, the metal ion-free (apo) form. The largest structural differences are seen, in the C-terminal EF-hand, and involve changes in both metal ion, coordination and helix packing. The N-terminal calcium binding site is not, occupied by any metal ion in the magnesium and manganese structures, and, shows little structural deviation from the apo and calcium bound forms., 1H-NMR and UV spectroscopic studies at physiological ion concentrations, show that the C-terminal site of the protein is significantly populated by, magnesium at resting cell calcium levels, and that there is a negative, allosteric interaction between magnesium and calcium binding. Calcium, binding was found to occur with positive cooperativity at physiological, magnesium concentration.
+
<StructureSection load='1ig5' size='340' side='right'caption='[[1ig5]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ig5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5icb 5icb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IG5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IG5 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ig5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ig5 OCA], [https://pdbe.org/1ig5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ig5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ig5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ig5 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/S100G_BOVIN S100G_BOVIN]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/1ig5_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ig5 ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1IG5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entry 5ICB. Known structural/functional Site: <scene name='pdbsite=MUM:Mg+Binding+Ef-Hand+Loop'>MUM</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IG5 OCA].
+
*[[S100 proteins 3D structures|S100 proteins 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structural basis for the negative allostery between Ca(2+)- and Mg(2+)-binding in the intracellular Ca(2+)-receptor calbindin D9k., Andersson M, Malmendal A, Linse S, Ivarsson I, Forsen S, Svensson LA, Protein Sci. 1997 Jun;6(6):1139-47. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9194174 9194174]
+
[[Category: Bos taurus]]
[[Category: Bos taurus]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Andersson, E.M.]]
+
[[Category: Andersson EM]]
-
[[Category: Svensson, L.A.]]
+
[[Category: Svensson LA]]
-
[[Category: MG]]
+
-
[[Category: calcium-binding protein]]
+
-
[[Category: ef-hand]]
+
-
[[Category: magnesium binding]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:50:33 2008''
+

Current revision

BOVINE CALBINDIN D9K BINDING MG2+

PDB ID 1ig5

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ig5"
Personal tools