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1igv

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[[Image:1igv.gif|left|200px]]<br /><applet load="1igv" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1igv, resolution 1.85&Aring;" />
 
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'''BOVINE CALBINDIN D9K BINDING MN2+'''<br />
 
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==Overview==
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==BOVINE CALBINDIN D9K BINDING MN2+==
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The three-dimensional structures of the magnesium- and manganese-bound, forms of calbindin D9k were determined to 1.6 A and 1.9 A resolution, respectively, using X-ray crystallography. These two structures are nearly, identical but deviate significantly from both the calcium bound form and, the metal ion-free (apo) form. The largest structural differences are seen, in the C-terminal EF-hand, and involve changes in both metal ion, coordination and helix packing. The N-terminal calcium binding site is not, occupied by any metal ion in the magnesium and manganese structures, and, shows little structural deviation from the apo and calcium bound forms., 1H-NMR and UV spectroscopic studies at physiological ion concentrations, show that the C-terminal site of the protein is significantly populated by, magnesium at resting cell calcium levels, and that there is a negative, allosteric interaction between magnesium and calcium binding. Calcium, binding was found to occur with positive cooperativity at physiological, magnesium concentration.
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<StructureSection load='1igv' size='340' side='right'caption='[[1igv]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1igv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6icb 6icb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IGV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1igv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1igv OCA], [https://pdbe.org/1igv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1igv RCSB], [https://www.ebi.ac.uk/pdbsum/1igv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1igv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/S100G_BOVIN S100G_BOVIN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/1igv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1igv ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1IGV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entry 6ICB. Known structural/functional Site: <scene name='pdbsite=MSE:Mn+Binding+Ef-Hand+Loop'>MSE</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IGV OCA].
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*[[S100 proteins 3D structures|S100 proteins 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural basis for the negative allostery between Ca(2+)- and Mg(2+)-binding in the intracellular Ca(2+)-receptor calbindin D9k., Andersson M, Malmendal A, Linse S, Ivarsson I, Forsen S, Svensson LA, Protein Sci. 1997 Jun;6(6):1139-47. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9194174 9194174]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Andersson, E.M.]]
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[[Category: Andersson EM]]
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[[Category: MN]]
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[[Category: calcium-binding protein]]
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[[Category: ef-hand]]
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[[Category: manganese binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:50:34 2008''
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Current revision

BOVINE CALBINDIN D9K BINDING MN2+

PDB ID 1igv

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