1iho

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[[Image:1iho.gif|left|200px]]
 
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==CRYSTAL APO-STRUCTURE OF PANTOTHENATE SYNTHETASE FROM E. COLI==
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The line below this paragraph, containing "STRUCTURE_1iho", creates the "Structure Box" on the page.
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<StructureSection load='1iho' size='340' side='right'caption='[[1iho]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1iho]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IHO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IHO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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{{STRUCTURE_1iho| PDB=1iho | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iho OCA], [https://pdbe.org/1iho PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iho RCSB], [https://www.ebi.ac.uk/pdbsum/1iho PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iho ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PANC_ECOLI PANC_ECOLI] Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.<ref>PMID:357689</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ih/1iho_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1iho ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL APO-STRUCTURE OF PANTOTHENATE SYNTHETASE FROM E. COLI'''
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==See Also==
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*[[Pantothenate synthetase|Pantothenate synthetase]]
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== References ==
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==Overview==
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<references/>
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BACKGROUND: Pantothenate synthetase (EC 6.3.2.1) is the last enzyme of the pathway of pantothenate (vitamin B(5)) synthesis. It catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction. RESULTS: We describe the overexpression, purification, and crystal structure of recombinant pantothenate synthetase from E. coli. The structure was solved by a selenomethionine multiwavelength anomalous dispersion experiment and refined against native data to a final R(cryst) of 22.6% (R(free) = 24.9%) at 1.7 A resolution. The enzyme is dimeric, with two well-defined domains per protomer: the N-terminal domain, a Rossmann fold, contains the active site cavity, with the C-terminal domain forming a hinged lid. CONCLUSIONS: The N-terminal domain is structurally very similar to class I aminoacyl-tRNA synthetases and is thus a member of the cytidylyltransferase superfamily. This relationship has been used to suggest the location of the ATP and pantoate binding sites and the nature of hinge bending that leads to the ternary enzyme-pantoate-ATP complex.
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__TOC__
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</StructureSection>
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==About this Structure==
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1IHO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IHO OCA].
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==Reference==
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The crystal structure of E. coli pantothenate synthetase confirms it as a member of the cytidylyltransferase superfamily., von Delft F, Lewendon A, Dhanaraj V, Blundell TL, Abell C, Smith AG, Structure. 2001 May 9;9(5):439-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11377204 11377204]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Pantoate--beta-alanine ligase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Abell C]]
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[[Category: Abell, C.]]
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[[Category: Blundell TL]]
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[[Category: Blundell, T L.]]
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[[Category: Dhanaraj V]]
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[[Category: Delft, F von.]]
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[[Category: Lewendon A]]
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[[Category: Dhanaraj, V.]]
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[[Category: Smith A]]
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[[Category: Lewendon, A.]]
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[[Category: Von Delft F]]
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[[Category: Smith, A.]]
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[[Category: Apo]]
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[[Category: Dimer]]
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[[Category: Flexible domain]]
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[[Category: High]]
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[[Category: Ksmk]]
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[[Category: Multidomain]]
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[[Category: Rossman fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:00:39 2008''
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Current revision

CRYSTAL APO-STRUCTURE OF PANTOTHENATE SYNTHETASE FROM E. COLI

PDB ID 1iho

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