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| - | [[Image:1j7c.gif|left|200px]] | |
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| - | <!-- | + | ==STRUCTURE OF THE ANABAENA FERREDOXIN MUTANT E95K== |
| - | The line below this paragraph, containing "STRUCTURE_1j7c", creates the "Structure Box" on the page.
| + | <StructureSection load='1j7c' size='340' side='right'caption='[[1j7c]], [[Resolution|resolution]] 1.80Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1j7c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1qoe 1qoe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J7C FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | --> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr> |
| - | {{STRUCTURE_1j7c| PDB=1j7c | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j7c OCA], [https://pdbe.org/1j7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j7c RCSB], [https://www.ebi.ac.uk/pdbsum/1j7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j7c ProSAT]</span></td></tr> |
| | + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/FER1_NOSS1 FER1_NOSS1] Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. |
| | + | == Evolutionary Conservation == |
| | + | [[Image:Consurf_key_small.gif|200px|right]] |
| | + | Check<jmol> |
| | + | <jmolCheckbox> |
| | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j7/1j7c_consurf.spt"</scriptWhenChecked> |
| | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | + | <text>to colour the structure by Evolutionary Conservation</text> |
| | + | </jmolCheckbox> |
| | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j7c ConSurf]. |
| | + | <div style="clear:both"></div> |
| | | | |
| - | '''STRUCTURE OF THE ANABAENA FERREDOXIN MUTANT E95K'''
| + | ==See Also== |
| - | | + | *[[Ferredoxin 3D structures|Ferredoxin 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | A combination of structural, thermodynamic, and transient kinetic data on wild-type and mutant Anabaena vegetative cell ferredoxins has been used to investigate the nature of the protein-protein interactions leading to electron transfer from reduced ferredoxin to oxidized ferredoxin:NADP+ reductase (FNR). We have determined the reduction potentials of wild-type vegetative ferredoxin, heterocyst ferredoxin, and 12 site-specific mutants at seven surface residues of vegetative ferredoxin, as well as the one- and two-electron reduction potentials of FNR, both alone and in complexes with wild-type and three mutant ferredoxins. X-ray crystallographic structure determinations have been carried out for six of the ferredoxin mutants. None of the mutants showed significant structural changes in the immediate vicinity of the [2Fe-2S] cluster, despite large decreases in electron-transfer reactivity (for E94K and S47A) and sizable increases in reduction potential (80 mV for E94K and 47 mV for S47A). Furthermore, the relatively small changes in Calpha backbone atom positions which were observed in these mutants do not correlate with the kinetic and thermodynamic properties. In sharp contrast to the S47A mutant, S47T retains electron-transfer activity, and its reduction potential is 100 mV more negative than that of the S47A mutant, implicating the importance of the hydrogen bond which exists between the side chain hydroxyl group of S47 and the side chain carboxyl oxygen of E94. Other ferredoxin mutations that alter both reduction potential and electron-transfer reactivity are E94Q, F65A, and F65I, whereas D62K, D68K, Q70K, E94D, and F65Y have reduction potentials and electron-transfer reactivity that are similar to those of wild-type ferredoxin. In electrostatic complexes with recombinant FNR, three of the kinetically impaired ferredoxin mutants, as did wild-type ferredoxin, induced large (approximately 40 mV) positive shifts in the reduction potential of the flavoprotein, thereby making electron transfer thermodynamically feasible. On the basis of these observations, we conclude that nonconservative mutations of three critical residues (S47, F65, and E94) on the surface of ferredoxin have large parallel effects on both the reduction potential and the electron-transfer reactivity of the [2Fe-2S] cluster and that the reduction potential changes are not the principal factor governing electron-transfer reactivity. Rather, the kinetic properties are most likely controlled by the specific orientations of the proteins within the transient electron-transfer complex.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Nostoc sp. PCC 7120 = FACHB-418]] |
| - | ==About this Structure==
| + | [[Category: Benning MM]] |
| - | 1J7C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1qoe 1qoe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J7C OCA].
| + | [[Category: Chae YK]] |
| - | | + | [[Category: Cheng H]] |
| - | ==Reference==
| + | [[Category: Gomez-Moreno C]] |
| - | Structure-function relationships in Anabaena ferredoxin: correlations between X-ray crystal structures, reduction potentials, and rate constants of electron transfer to ferredoxin:NADP+ reductase for site-specific ferredoxin mutants., Hurley JK, Weber-Main AM, Stankovich MT, Benning MM, Thoden JB, Vanhooke JL, Holden HM, Chae YK, Xia B, Cheng H, Markley JL, Martinez-Julvez M, Gomez-Moreno C, Schmeits JL, Tollin G, Biochemistry. 1997 Sep 16;36(37):11100-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9287153 9287153]
| + | [[Category: Holden HM]] |
| - | [[Category: Anabaena sp.]] | + | [[Category: Hurley JK]] |
| - | [[Category: Single protein]] | + | [[Category: Markley JL]] |
| - | [[Category: Benning, M M.]] | + | [[Category: Martinez-Julvez M]] |
| - | [[Category: Chae, Y K.]] | + | [[Category: Schmeits JL]] |
| - | [[Category: Cheng, H.]] | + | [[Category: Stankovich MT]] |
| - | [[Category: Gomez-Moreno, C.]] | + | [[Category: Thoden JB]] |
| - | [[Category: Holden, H M.]] | + | [[Category: Tollin G]] |
| - | [[Category: Hurley, J K.]] | + | [[Category: Vanhooke JL]] |
| - | [[Category: Markley, J L.]] | + | [[Category: Weber-Main AM]] |
| - | [[Category: Martinez-Julvez, M.]] | + | [[Category: Xia B]] |
| - | [[Category: Schmeits, J L.]] | + | |
| - | [[Category: Stankovich, M T.]] | + | |
| - | [[Category: Thoden, J B.]] | + | |
| - | [[Category: Tollin, G.]] | + | |
| - | [[Category: Vanhooke, J L.]] | + | |
| - | [[Category: Weber-Main, A M.]] | + | |
| - | [[Category: Xia, B.]] | + | |
| - | [[Category: Electron transport]]
| + | |
| - | [[Category: Ferredoxin]]
| + | |
| - | [[Category: Iron-sulfur]]
| + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:52:26 2008''
| + | |
