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1j90

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Crystal Structure of Drosophila Deoxyribonucleoside Kinase

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Retrieved from "http://52.214.119.220/wiki/index.php/1j90"

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-[[Image:1j90.jpg|left|200px]]
- {{Structure
+==Crystal Structure of Drosophila Deoxyribonucleoside Kinase==
-|PDB= 1j90 |SIZE=350|CAPTION= <scene name='initialview01'>1j90</scene>, resolution 2.56&Aring;
+<StructureSection load='1j90' size='340' side='right'caption='[[1j90]], [[Resolution|resolution]] 2.56&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=DCZ:2&#39;-DEOXYCYTIDINE'>DCZ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+<table><tr><td colspan='2'>[[1j90]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J90 FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.56&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DCZ:2-DEOXYCYTIDINE'>DCZ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j90 OCA], [https://pdbe.org/1j90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j90 RCSB], [https://www.ebi.ac.uk/pdbsum/1j90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j90 ProSAT]</span></td></tr>
-|RELATEDENTRY=
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j90 OCA], [http://www.ebi.ac.uk/pdbsum/1j90 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1j90 RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/DNK_DROME DNK_DROME] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.<ref>PMID:10446143</ref> <ref>PMID:10692477</ref> <ref>PMID:16008571</ref>
+== Evolutionary Conservation ==
-'''Crystal Structure of Drosophila Deoxyribonucleoside Kinase'''
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
-==Overview==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j9/1j90_consurf.spt"</scriptWhenChecked>
-Deoxyribonucleoside kinases phosphorylate deoxyribonucleosides and activate a number of medically important nucleoside analogs. Here we report the structure of the Drosophila deoxyribonucleoside kinase with deoxycytidine bound at the nucleoside binding site and that of the human deoxyguanosine kinase with ATP at the nucleoside substrate binding site. Compared to the human kinase, the Drosophila kinase has a wider substrate cleft, which may be responsible for the broad substrate specificity of this enzyme. The human deoxyguanosine kinase is highly specific for purine substrates; this is apparently due to the presence of Arg 118, which provides favorable hydrogen bonding interactions with the substrate. The two new structures provide an explanation for the substrate specificity of cellular deoxyribonucleoside kinases.
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==About this Structure==
+  </jmolCheckbox>
-J90 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J90 OCA].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j90 ConSurf].
+<div style="clear:both"></div>
-==Reference==
+== References ==
-Structural basis for substrate specificities of cellular deoxyribonucleoside kinases., Johansson K, Ramaswamy S, Ljungcrantz C, Knecht W, Piskur J, Munch-Petersen B, Eriksson S, Eklund H, Nat Struct Biol. 2001 Jul;8(7):616-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11427893 11427893]
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Drosophila melanogaster]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Eklund, H.]]
+[[Category: Eklund H]]
-[[Category: Eriksson, S.]]
+[[Category: Eriksson S]]
-[[Category: Johansson, K.]]
+[[Category: Johansson K]]
-[[Category: Knecht, W.]]
+[[Category: Knecht W]]
-[[Category: Ljungkrantz, C.]]
+[[Category: Ljungkrantz C]]
-[[Category: Munch-Petersen, B.]]
+[[Category: Munch-Petersen B]]
-[[Category: Piskur, J.]]
+[[Category: Piskur J]]
-[[Category: Ramaswamy, S.]]
+[[Category: Ramaswamy S]]
-[[Category: protein-deoxynucleoside complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:30:17 2008''

1j90

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Crystal Structure of Drosophila Deoxyribonucleoside Kinase

Structural highlights

Function

Evolutionary Conservation

References

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