1jch

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[[Image:1jch.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of Colicin E3 in Complex with its Immunity Protein==
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|PDB= 1jch |SIZE=350|CAPTION= <scene name='initialview01'>1jch</scene>, resolution 3.02&Aring;
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<StructureSection load='1jch' size='340' side='right'caption='[[1jch]], [[Resolution|resolution]] 3.02&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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<table><tr><td colspan='2'>[[1jch]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._W3110 Escherichia coli str. K-12 substr. W3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JCH FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.02&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jch OCA], [https://pdbe.org/1jch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jch RCSB], [https://www.ebi.ac.uk/pdbsum/1jch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jch ProSAT]</span></td></tr>
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|RELATEDENTRY=[[3eip|3EIP]], [[1e44|1E44]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jch OCA], [http://www.ebi.ac.uk/pdbsum/1jch PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jch RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/CEA3_ECOLX CEA3_ECOLX] Inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes at a specific site. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jc/1jch_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jch ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of Colicin E3 in Complex with its Immunity Protein'''
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==See Also==
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*[[Colicin 3D structures|Colicin 3D structures]]
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*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
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==Overview==
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__TOC__
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Colicins kill E. coli by a process that involves binding to a surface receptor, entering the cell, and, finally, intoxicating it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3, reported here in a binary complex with its immunity protein (IP), reveals a Y-shaped molecule with the receptor binding domain forming a 100 A long stalk and the two globular heads of the translocation domain (T) and the catalytic domain (C) comprising the two arms. Active site residues are D510, H513, E517, and R545. IP is buried between T and C. Rather than blocking the active site, IP prevents access of the active site to the ribosome.
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</StructureSection>
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[[Category: Escherichia coli str. K-12 substr. W3110]]
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==About this Structure==
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[[Category: Large Structures]]
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1JCH is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JCH OCA].
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[[Category: Jakes K]]
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[[Category: Li C]]
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==Reference==
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[[Category: Shoham M]]
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Crystal structure of colicin E3: implications for cell entry and ribosome inactivation., Soelaiman S, Jakes K, Wu N, Li C, Shoham M, Mol Cell. 2001 Nov;8(5):1053-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11741540 11741540]
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[[Category: Soelaiman S]]
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[[Category: Escherichia coli]]
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[[Category: Wu N]]
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[[Category: Protein complex]]
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[[Category: Jakes, K.]]
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[[Category: Li, C.]]
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[[Category: Shoham, M.]]
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[[Category: Soelaiman, S.]]
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[[Category: Wu, N.]]
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[[Category: the receptor-binding domain is a coiled coil]]
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[[Category: the rnase domain is a six-stranded antiparallel beta-sheet. the immunity protein is a four-stranded antiparallel beta sheet flanked by 3 helices on one side of the sheet]]
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[[Category: translocation domain is a beta-jellyroll]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:31:42 2008''
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Current revision

Crystal Structure of Colicin E3 in Complex with its Immunity Protein

PDB ID 1jch

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