1jch

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(New page: 200px<br /><applet load="1jch" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jch, resolution 3.02&Aring;" /> '''Crystal Structure of...)
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[[Image:1jch.gif|left|200px]]<br /><applet load="1jch" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jch, resolution 3.02&Aring;" />
 
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'''Crystal Structure of Colicin E3 in Complex with its Immunity Protein'''<br />
 
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==Overview==
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==Crystal Structure of Colicin E3 in Complex with its Immunity Protein==
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Colicins kill E. coli by a process that involves binding to a surface, receptor, entering the cell, and, finally, intoxicating it. The lethal, action of colicin E3 is a specific cleavage in the ribosomal decoding A, site. The crystal structure of colicin E3, reported here in a binary, complex with its immunity protein (IP), reveals a Y-shaped molecule with, the receptor binding domain forming a 100 A long stalk and the two, globular heads of the translocation domain (T) and the catalytic domain, (C) comprising the two arms. Active site residues are D510, H513, E517, and R545. IP is buried between T and C. Rather than blocking the active, site, IP prevents access of the active site to the ribosome.
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<StructureSection load='1jch' size='340' side='right'caption='[[1jch]], [[Resolution|resolution]] 3.02&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jch]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._W3110 Escherichia coli str. K-12 substr. W3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JCH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.02&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jch OCA], [https://pdbe.org/1jch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jch RCSB], [https://www.ebi.ac.uk/pdbsum/1jch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jch ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CEA3_ECOLX CEA3_ECOLX] Inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes at a specific site. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jc/1jch_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jch ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JCH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CIT and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JCH OCA].
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*[[Colicin 3D structures|Colicin 3D structures]]
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*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
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==Reference==
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__TOC__
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Crystal structure of colicin E3: implications for cell entry and ribosome inactivation., Soelaiman S, Jakes K, Wu N, Li C, Shoham M, Mol Cell. 2001 Nov;8(5):1053-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11741540 11741540]
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Escherichia coli str. K-12 substr. W3110]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Jakes, K.]]
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[[Category: Jakes K]]
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[[Category: Li, C.]]
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[[Category: Li C]]
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[[Category: Shoham, M.]]
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[[Category: Shoham M]]
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[[Category: Soelaiman, S.]]
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[[Category: Soelaiman S]]
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[[Category: Wu, N.]]
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[[Category: Wu N]]
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[[Category: CIT]]
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[[Category: GOL]]
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[[Category: the receptor-binding domain is a coiled coil]]
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[[Category: the rnase domain is a six-stranded antiparallel beta-sheet. the immunity protein is a four-stranded antiparallel beta sheet flanked by 3 helices on one side of the sheet]]
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[[Category: translocation domain is a beta-jellyroll]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:06:25 2007''
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Current revision

Crystal Structure of Colicin E3 in Complex with its Immunity Protein

PDB ID 1jch

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