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1jf8

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[[Image:1jf8.gif|left|200px]]<br /><applet load="1jf8" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jf8, resolution 1.12&Aring;" />
 
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'''X-ray structure of reduced C10S, C15A arsenate reductase from pI258'''<br />
 
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==Overview==
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==X-ray structure of reduced C10S, C15A arsenate reductase from pI258==
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Arsenate reductase (ArsC) from Staphylococcus aureus plasmid pI258 plays a role in bacterial heavy metal resistance and catalyzes the reduction of arsenate to arsenite. The structures of the oxidized and reduced forms of ArsC were solved. ArsC has the PTPase I fold typical for low molecular weight tyrosine phosphatases (LMW PTPases). Remarkably, kinetic experiments show that pI258 ArsC also catalyzes the tyrosine phosphatase reaction in addition to arsenate reduction. These results provide evidence that ArsC from pI258 evolved from LMW PTPase by the grafting of a redox function onto a pre-existing catalytic site and that its evolutionary origin is different from those of arsenate reductases from Escherichia coli plasmid R773 and from Saccharomyces cerevisiae. The mechanism proposed here for the catalysis of arsenate reduction by pI258 ArsC involves a nucleophilic attack by Cys 10 on arsenate, the formation of a covalent intermediate and the transport of oxidative equivalents by a disulfide cascade. The reaction is associated with major structural changes in the ArsC.
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<StructureSection load='1jf8' size='340' side='right'caption='[[1jf8]], [[Resolution|resolution]] 1.12&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jf8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JF8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.12&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jf8 OCA], [https://pdbe.org/1jf8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jf8 RCSB], [https://www.ebi.ac.uk/pdbsum/1jf8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jf8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARSC_STAAU ARSC_STAAU] Reduces arsenate [As(V)] to arsenite [As(III)] and dephosphorylates tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Could switch between different functions in different circumstances.[HAMAP-Rule:MF_01624]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jf/1jf8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jf8 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JF8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=BCT:'>BCT</scene>, <scene name='pdbligand=K:'>K</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JF8 OCA].
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*[[Arsenate reductase 3D structures|Arsenate reductase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Arsenate reductase from S. aureus plasmid pI258 is a phosphatase drafted for redox duty., Zegers I, Martins JC, Willem R, Wyns L, Messens J, Nat Struct Biol. 2001 Oct;8(10):843-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11573087 11573087]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Martins, J C.]]
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[[Category: Martins JC]]
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[[Category: Messens, J.]]
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[[Category: Messens J]]
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[[Category: Willem, R.]]
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[[Category: Willem R]]
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[[Category: Wyns, L.]]
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[[Category: Wyns L]]
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[[Category: Zegers, I.]]
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[[Category: Zegers I]]
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[[Category: BCT]]
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[[Category: K]]
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[[Category: TRS]]
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[[Category: p-loop]]
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[[Category: ptpase i fold]]
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[[Category: sulfinic acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:21:58 2008''
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Current revision

X-ray structure of reduced C10S, C15A arsenate reductase from pI258

PDB ID 1jf8

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