This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.


Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1jgg

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:39, 7 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1jgg.gif|left|200px]]<br /><applet load="1jgg" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1jgg, resolution 2.&Aring;" />
 
-
'''Even-skipped Homeodomain Complexed to AT-rich DNA'''<br />
 
-
==Overview==
+
==Even-skipped Homeodomain Complexed to AT-rich DNA==
-
even-skipped is a homeobox gene important in controlling segment patterning in the embryonic fruit fly. Its homeobox encodes a DNA binding domain which binds with similar affinities to two DNA consensus sequences, one AT-rich, the other GC-rich. We describe a crystallographic analysis of the Even-skipped homeodomain complexed to an AT-rich oligonucleotide at 2.0 A resolution. The structure reveals a novel arrangement of two homeodomains bound to one 10 bp DNA sequence in a tandem fashion. This arrangement suggests a mechanism for the homeoproteins' regulatory specificity. In addition, the functionally important residue Gln50 is observed in multiple conformations making direct and water-mediated hydrogen bonds with the DNA bases.
+
<StructureSection load='1jgg' size='340' side='right'caption='[[1jgg]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1jgg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JGG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JGG FirstGlance]. <br>
-
1JGG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JGG OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jgg OCA], [https://pdbe.org/1jgg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jgg RCSB], [https://www.ebi.ac.uk/pdbsum/1jgg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jgg ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Structure of the even-skipped homeodomain complexed to AT-rich DNA: new perspectives on homeodomain specificity., Hirsch JA, Aggarwal AK, EMBO J. 1995 Dec 15;14(24):6280-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8557047 8557047]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/EVE_DROME EVE_DROME] May play a role in determining neuronal identity. May be directly involved in specifying identity of individual neurons. Pair-rule protein required for segmentation; involved in transforming the broad, spatial, aperiodic expression patterns of the gap genes into a system of precise periodic expression patterns of the pair-rule and segmentary polarity genes.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jg/1jgg_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jgg ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Aggarwal, A K.]]
+
[[Category: Aggarwal AK]]
-
[[Category: Hirsch, J A.]]
+
[[Category: Hirsch JA]]
-
[[Category: even-skipped]]
+
-
[[Category: homeodomain]]
+
-
[[Category: protein-dna complex]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:21 2008''
+

Current revision

Even-skipped Homeodomain Complexed to AT-rich DNA

PDB ID 1jgg

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools