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1jkm

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[[Image:1jkm.gif|left|200px]]<br /><applet load="1jkm" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jkm, resolution 1.85&Aring;" />
 
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'''BREFELDIN A ESTERASE, A BACTERIAL HOMOLOGUE OF HUMAN HORMONE SENSITIVE LIPASE'''<br />
 
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==Overview==
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==BREFELDIN A ESTERASE, A BACTERIAL HOMOLOGUE OF HUMAN HORMONE SENSITIVE LIPASE==
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Brefeldin A esterase (BFAE), a detoxifying enzyme isolated from Bacillus subtilis, hydrolyzes and inactivates BFA, a potent fungal inhibitor of intracellular vesicle-dependent secretory transport and poliovirus RNA replication. We have solved the crystal structure of BFAE and we discovered that the previously reported amino acid sequence was in serious error due to frame shifts in the cDNA sequence. The correct sequence, inferred from the experimentally phased electron density map, revealed that BFAE is a homolog of the mammalian hormone sensitive lipase (HSL). It is a canonical alpha/beta hydrolase with two insertions forming the substrate binding pocket. The enzyme contains a lipase-like catalytic triad, Ser 202, Asp 308 and His 338, consistent with mutational studies that implicate the homologous Ser 424, Asp 693 and His 723 in the catalytic triad in human HSL.
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<StructureSection load='1jkm' size='340' side='right'caption='[[1jkm]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1jkm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JKM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JKM FirstGlance]. <br>
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1JKM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JKM OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jkm OCA], [https://pdbe.org/1jkm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jkm RCSB], [https://www.ebi.ac.uk/pdbsum/1jkm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jkm ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of brefeldin A esterase, a bacterial homolog of the mammalian hormone-sensitive lipase., Wei Y, Contreras JA, Sheffield P, Osterlund T, Derewenda U, Kneusel RE, Matern U, Holm C, Derewenda ZS, Nat Struct Biol. 1999 Apr;6(4):340-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10201402 10201402]
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== Function ==
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[https://www.uniprot.org/uniprot/O68884_BACIU O68884_BACIU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jk/1jkm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jkm ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Contreras, A J.]]
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[[Category: Contreras AJ]]
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[[Category: Derewenda, U.]]
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[[Category: Derewenda U]]
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[[Category: Derewenda, Z S.]]
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[[Category: Derewenda ZS]]
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[[Category: Matern, U O.]]
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[[Category: Matern UO]]
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[[Category: Osterlund, T.]]
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[[Category: Osterlund T]]
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[[Category: Sheffield, P.]]
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[[Category: Sheffield P]]
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[[Category: Wei, Y.]]
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[[Category: Wei Y]]
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[[Category: alpha/beta hydrolase family]]
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[[Category: degradation of brefeldin a]]
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[[Category: serine hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:23:42 2008''
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Current revision

BREFELDIN A ESTERASE, A BACTERIAL HOMOLOGUE OF HUMAN HORMONE SENSITIVE LIPASE

PDB ID 1jkm

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