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1jlv

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[[Image:1jlv.gif|left|200px]]<br /><applet load="1jlv" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jlv, resolution 1.75&Aring;" />
 
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'''Anopheles dirus species B glutathione S-transferases 1-3'''<br />
 
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==Overview==
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==Anopheles dirus species B glutathione S-transferases 1-3==
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Glutathione S-transferases (GSTs) are dimeric proteins that play an important role in cellular detoxification. Four GSTs from the mosquito Anopheles dirus species B (Ad), an important malaria vector in South East Asia, are produced by alternate splicing of a single transcription product and were previously shown to have detoxifying activity towards pesticides such as DDT. We have determined the crystal structures for two of these alternatively spliced proteins, AdGST1-3 (complexed with glutathione) and AdGST1-4 (apo form), at 1.75 and 2.45 A resolution, respectively. These GST isozymes show differences from the related GST from the Australian sheep blowfly Lucilia cuprina; in particular, the presence of a C-terminal helix forming part of the active site. This helix causes the active site of the Anopheles GSTs to be enclosed. The glutathione-binding helix alpha2 and flanking residues are disordered in the AdGST1-4 (apo) structure, yet ordered in the AdGST1-3 (GSH-bound) structure, suggesting that insect GSTs operate with an induced fit mechanism similar to that found in the plant phi- and human pi-class GSTs. Despite the high overall sequence identities, the active site residues of AdGST1-4 and AdGST1-3 have different conformations.
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<StructureSection load='1jlv' size='340' side='right'caption='[[1jlv]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jlv]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Anopheles_cracens Anopheles cracens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JLV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JLV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jlv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jlv OCA], [https://pdbe.org/1jlv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jlv RCSB], [https://www.ebi.ac.uk/pdbsum/1jlv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jlv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7KIF2_9DIPT Q7KIF2_9DIPT]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jl/1jlv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jlv ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JLV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anopheles_cracens Anopheles cracens] with <scene name='pdbligand=GSH:'>GSH</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JLV OCA].
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*[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystal structures of glutathione S-transferases isozymes 1-3 and 1-4 from Anopheles dirus species B., Oakley AJ, Harnnoi T, Udomsinprasert R, Jirajaroenrat K, Ketterman AJ, Wilce MC, Protein Sci. 2001 Nov;10(11):2176-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11604524 11604524]
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[[Category: Anopheles cracens]]
[[Category: Anopheles cracens]]
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[[Category: Glutathione transferase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Harnnoi T]]
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[[Category: Harnnoi, T.]]
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[[Category: Jirajaroenrat K]]
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[[Category: Jirajaroenrat, K.]]
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[[Category: Ketterman AJ]]
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[[Category: Ketterman, A J.]]
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[[Category: Oakley AJ]]
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[[Category: Oakley, A J.]]
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[[Category: Udomsinprasert R]]
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[[Category: Udomsinprasert, R.]]
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[[Category: Wilce MC]]
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[[Category: Wilce, M C.]]
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[[Category: GSH]]
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[[Category: adgst1-3]]
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[[Category: glutathione s-transferase]]
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[[Category: gst]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:12 2008''
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Current revision

Anopheles dirus species B glutathione S-transferases 1-3

PDB ID 1jlv

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