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1jot

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[[Image:1jot.gif|left|200px]]
 
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==STRUCTURE OF THE LECTIN MPA COMPLEXED WITH T-ANTIGEN DISACCHARIDE==
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The line below this paragraph, containing "STRUCTURE_1jot", creates the "Structure Box" on the page.
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<StructureSection load='1jot' size='340' side='right'caption='[[1jot]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1jot]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Maclura_pomifera Maclura pomifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JOT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JOT FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900084:Thomsen-Friedenreich+antigen'>PRD_900084</scene></td></tr>
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{{STRUCTURE_1jot| PDB=1jot | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jot OCA], [https://pdbe.org/1jot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jot RCSB], [https://www.ebi.ac.uk/pdbsum/1jot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jot ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LECA_MACPO LECA_MACPO] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jo/1jot_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jot ConSurf].
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<div style="clear:both"></div>
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'''STRUCTURE OF THE LECTIN MPA COMPLEXED WITH T-ANTIGEN DISACCHARIDE'''
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==See Also==
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*[[Agglutinin 3D structures|Agglutinin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Maclura pomifera agglutinin is a tetrameric plant seed lectin with high affinity for the tumor-associated T-antigen disaccharide, Galbeta1,3GalNAcalpha, and hence for many O-linked glycopeptide structures. Unlike members of most lectin families, it lacks both metal ions and Cys residues. The structure of its complex with Galbeta1,3GalNAc was determined to 2.2 by first using multiwavelength anomalous diffraction with a lead derivative of the native protein, and then using molecular replacement with the unrefined structure as a model to solve the structure of the complex. The subunits share the beta-prism architecture and three-fold pseudo-symmetry of the related lectin jacalin, with the 21-residue beta-chains in the center of the tetramer. Interactions with the GalNAc predominate in the binding of the disaccharide. It forms a network of H-bonds with only one side chain, from an Asp residue, the amino group of the N-terminal Gly of the alpha-chain, and peptide backbone atoms of two aromatic residues. The Gal moiety does not H-bond directly with residues in the same monomer, i.e. there is no true subsite for it, but there are interactions through two water molecules. In the crystal, it interacts with residues in the binding site of an adjacent tetramer. The minimum energy conformation expected for the disaccharide is retained, despite its mediating the tetramer-tetramer interactions in the crystal packing. The resulting lattice is comparable to those seen for complexes of other lectins with branched glycopeptides.
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[[Category: Large Structures]]
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==About this Structure==
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1JOT is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Maclura_pomifera Maclura pomifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JOT OCA].
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==Reference==
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Structure of the complex of Maclura pomifera agglutinin and the T-antigen disaccharide, Galbeta1,3GalNAc., Lee X, Thompson A, Zhang Z, Ton-that H, Biesterfeldt J, Ogata C, Xu L, Johnston RA, Young NM, J Biol Chem. 1998 Mar 13;273(11):6312-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9497359 9497359]
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[[Category: Maclura pomifera]]
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[[Category: Protein complex]]
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[[Category: Biesterfeldt, J.]]
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[[Category: Hoa, T T.]]
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[[Category: Johnston, R A.Z.]]
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[[Category: Lee, X.]]
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[[Category: Ogata, C.]]
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[[Category: Thompson, A.]]
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[[Category: Xu, L.]]
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[[Category: Young, N M.]]
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[[Category: Zhang, Z.]]
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[[Category: Beta prism]]
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[[Category: Lectin]]
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[[Category: Maclura pomifera]]
[[Category: Maclura pomifera]]
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[[Category: T-antigen]]
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[[Category: Biesterfeldt J]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:31:34 2008''
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[[Category: Hoa T-T]]
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[[Category: Johnston RAZ]]
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[[Category: Lee X]]
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[[Category: Ogata C]]
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[[Category: Thompson A]]
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[[Category: Xu L]]
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[[Category: Young NM]]
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[[Category: Zhang Z]]

Current revision

STRUCTURE OF THE LECTIN MPA COMPLEXED WITH T-ANTIGEN DISACCHARIDE

PDB ID 1jot

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