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| - | [[Image:1juc.gif|left|200px]] | |
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| - | {{Structure
| + | ==Crystal Structure Analysis of a Holliday Junction Formed by CCGGTACCGG== |
| - | |PDB= 1juc |SIZE=350|CAPTION= <scene name='initialview01'>1juc</scene>, resolution 2.35Å
| + | <StructureSection load='1juc' size='340' side='right'caption='[[1juc]], [[Resolution|resolution]] 2.35Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[1juc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JUC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JUC FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1juc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1juc OCA], [https://pdbe.org/1juc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1juc RCSB], [https://www.ebi.ac.uk/pdbsum/1juc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1juc ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1juc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1juc OCA], [http://www.ebi.ac.uk/pdbsum/1juc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1juc RCSB]</span>
| + | </StructureSection> |
| - | }}
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Cardin CJ]] |
| - | '''Crystal Structure Analysis of a Holliday Junction Formed by CCGGTACCGG'''
| + | [[Category: Gale BC]] |
| - | | + | [[Category: Teixeira SCM]] |
| - | | + | [[Category: Thorpe JH]] |
| - | ==Overview==
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| - | DNA-strand exchange is a vital step in the recombination process, of which a key intermediate is the four-way DNA Holliday junction formed transiently in most living organisms. Here, the single-crystal structure at a resolution of 2.35 A of such a DNA junction formed by d(CCGGTACCGG)(2), which has crystallized in a more highly symmetrical packing mode to that previously observed for the same sequence, is presented. In this case, the structure is isomorphous to the mismatch sequence d(CCGGGACCGG)(2), which reveals the roles of both lattice and DNA sequence in determining the junction geometry. The helices cross at the larger angle of 43.0 degrees (the previously observed angle for this sequence was 41.4 degrees) as a right-handed X. No metal cations were observed; the crystals were grown in the presence of only group I counter-cations.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1JUC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JUC OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Structural characterization of a new crystal form of the four-way Holliday junction formed by the DNA sequence d(CCGGTACCGG)2: sequence versus lattice?, Thorpe JH, Teixeira SC, Gale BC, Cardin CJ, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):567-9. Epub 2002, Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11856858 11856858]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Cardin, C J.]] | + | |
| - | [[Category: Gale, B C.]] | + | |
| - | [[Category: Teixeira, S C.M.]] | + | |
| - | [[Category: Thorpe, J H.]] | + | |
| - | [[Category: holliday junction]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:39:07 2008''
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