1juc

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(New page: 200px<br /><applet load="1juc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1juc, resolution 2.35&Aring;" /> '''Crystal Structure An...)
Current revision (07:42, 7 February 2024) (edit) (undo)
 
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[[Image:1juc.gif|left|200px]]<br /><applet load="1juc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1juc, resolution 2.35&Aring;" />
 
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'''Crystal Structure Analysis of a Holliday Junction Formed by CCGGTACCGG'''<br />
 
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==Overview==
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==Crystal Structure Analysis of a Holliday Junction Formed by CCGGTACCGG==
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DNA-strand exchange is a vital step in the recombination process, of which, a key intermediate is the four-way DNA Holliday junction formed, transiently in most living organisms. Here, the single-crystal structure, at a resolution of 2.35 A of such a DNA junction formed by, d(CCGGTACCGG)(2), which has crystallized in a more highly symmetrical, packing mode to that previously observed for the same sequence, is, presented. In this case, the structure is isomorphous to the mismatch, sequence d(CCGGGACCGG)(2), which reveals the roles of both lattice and DNA, sequence in determining the junction geometry. The helices cross at the, larger angle of 43.0 degrees (the previously observed angle for this, sequence was 41.4 degrees) as a right-handed X. No metal cations were, observed; the crystals were grown in the presence of only group I, counter-cations.
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<StructureSection load='1juc' size='340' side='right'caption='[[1juc]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1juc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JUC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JUC FirstGlance]. <br>
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1JUC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JUC OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1juc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1juc OCA], [https://pdbe.org/1juc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1juc RCSB], [https://www.ebi.ac.uk/pdbsum/1juc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1juc ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structural characterization of a new crystal form of the four-way Holliday junction formed by the DNA sequence d(CCGGTACCGG)2: sequence versus lattice?, Thorpe JH, Teixeira SC, Gale BC, Cardin CJ, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):567-9. Epub 2002, Feb 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11856858 11856858]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Cardin, C.J.]]
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[[Category: Large Structures]]
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[[Category: Gale, B.C.]]
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[[Category: Cardin CJ]]
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[[Category: Teixeira, S.C.M.]]
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[[Category: Gale BC]]
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[[Category: Thorpe, J.H.]]
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[[Category: Teixeira SCM]]
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[[Category: holliday junction]]
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[[Category: Thorpe JH]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:15:56 2007''
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Current revision

Crystal Structure Analysis of a Holliday Junction Formed by CCGGTACCGG

PDB ID 1juc

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