This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1k02

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:43, 7 February 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1k02.jpg|left|200px]]
 
-
{{Structure
+
==Crystal Structure of Old Yellow Enzyme Mutant Gln114Asn==
-
|PDB= 1k02 |SIZE=350|CAPTION= <scene name='initialview01'>1k02</scene>, resolution 2.7&Aring;
+
<StructureSection load='1k02' size='340' side='right'caption='[[1k02]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+
<table><tr><td colspan='2'>[[1k02]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K02 FirstGlance]. <br>
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k02 OCA], [https://pdbe.org/1k02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k02 RCSB], [https://www.ebi.ac.uk/pdbsum/1k02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k02 ProSAT]</span></td></tr>
-
|RELATEDENTRY=[[1oya|1OYA]], [[1oyb|1OYB]], [[1oyc|1OYC]], [[1k03|1K03]]
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k02 OCA], [http://www.ebi.ac.uk/pdbsum/1k02 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k02 RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/OYE1_SACPS OYE1_SACPS] Oxidizes beta-NADH, beta-NADPH, and alpha-NADPH.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/1k02_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k02 ConSurf].
 +
<div style="clear:both"></div>
-
'''Crystal Structure of Old Yellow Enzyme Mutant Gln114Asn'''
+
==See Also==
-
 
+
*[[NADPH dehydrogenase|NADPH dehydrogenase]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
Glutamine 114 of OYE1 is a well conserved residue in the active site of the Old Yellow Enzyme family. It forms hydrogen bonds to the O2 and N3 of the flavoprotein prosthetic group, FMN. Glutamine 114 was mutated to asparagine, introducing an R-group that is one methylene group shorter. The resultant enzyme was characterized to determine the effect of the mutation on the mechanistic behavior of the enzyme, and the crystal structure was solved to determine the effect of the mutation on the structure of the protein. The Q114N mutation results in little change in the protein structure, moving the amide group of residue 114 out of H-bonding distance, allowing repositioning of the FMN prosthetic group to form new interactions that replace the lost H-bonds. The mutation decreases the ability to bind ligands, as all dissociation constants for substituted phenols are larger than for the wild type enzyme. The rate constant for the reductive half-reaction with beta-NADPH is slightly greater, whereas that for the oxidative half-reaction with 2-cyclohexenone is smaller than for the wild type enzyme. Oxidation with molecular oxygen is biphasic and involves formation and reaction with O(2), a phenomenon that is more pronounced with this mutation than with wild type enzyme. When superoxide dismutase is added to the reaction, we observe a single-phase reaction typical of the wild type enzyme. Turnover reactions using beta-NADPH with 2-cyclohexenone and molecular oxygen were studied to further characterize the mutant enzyme.
+
[[Category: Large Structures]]
-
 
+
-
==About this Structure==
+
-
1K02 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K02 OCA].
+
-
 
+
-
==Reference==
+
-
The role of glutamine 114 in old yellow enzyme., Brown BJ, Hyun JW, Duvvuri S, Karplus PA, Massey V, J Biol Chem. 2002 Jan 18;277(3):2138-45. Epub 2001 Oct 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11668181 11668181]
+
-
[[Category: NADPH dehydrogenase]]
+
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Single protein]]
+
[[Category: Brown BJ]]
-
[[Category: Brown, B J.]]
+
[[Category: Duvvuri SD]]
-
[[Category: Duvvuri, S D.]]
+
[[Category: Hyun J]]
-
[[Category: Hyun, J.]]
+
[[Category: Karplus PA]]
-
[[Category: Karplus, P A.]]
+
[[Category: Massey V]]
-
[[Category: Massey, V.]]
+
-
[[Category: beta-alpha barrel]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:41:26 2008''
+

Current revision

Crystal Structure of Old Yellow Enzyme Mutant Gln114Asn

PDB ID 1k02

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1k02"
Personal tools