This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1k0n

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1k0n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k0n, resolution 1.80&Aring;" /> '''Chloride Intracellu...)
Current revision (07:43, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1k0n.gif|left|200px]]<br />
 
-
<applet load="1k0n" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1k0n, resolution 1.80&Aring;" />
 
-
'''Chloride Intracellular Channel 1 (CLIC1) complexed with glutathione'''<br />
 
-
==Overview==
+
==Chloride Intracellular Channel 1 (CLIC1) complexed with glutathione==
-
CLIC1 (NCC27) is a member of the highly conserved class of chloride ion, channels that exists in both soluble and integral membrane forms. Purified, CLIC1 can integrate into synthetic lipid bilayers forming a chloride, channel with similar properties to those observed in vivo. The structure, of the soluble form of CLIC1 has been determined at 1.4-A resolution. The, protein is monomeric and structurally homologous to the glutathione, S-transferase superfamily, and it has a redox-active site resembling, glutaredoxin. The structure of the complex of CLIC1 with glutathione shows, that glutathione occupies the redox-active site, which is adjacent to an, open, elongated slot lined by basic residues. Integration of CLIC1 into, the membrane is likely to require a major structural rearrangement, probably of the N-domain (residues 1-90), with the putative transmembrane, helix arising from residues in the vicinity of the redox-active site. The, structure indicates that CLIC1 is likely to be controlled by, redox-dependent processes.
+
<StructureSection load='1k0n' size='340' side='right'caption='[[1k0n]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1k0n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K0N FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0n OCA], [https://pdbe.org/1k0n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k0n RCSB], [https://www.ebi.ac.uk/pdbsum/1k0n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0n ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CLIC1_HUMAN CLIC1_HUMAN] Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.<ref>PMID:9139710</ref> <ref>PMID:10834939</ref> <ref>PMID:11195932</ref> <ref>PMID:11940526</ref> <ref>PMID:11978800</ref> <ref>PMID:11551966</ref> <ref>PMID:14613939</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/1k0n_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0n ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1K0N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with GTT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K0N OCA].
+
*[[Ion channels 3D structures|Ion channels 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
Crystal structure of a soluble form of the intracellular chloride ion channel CLIC1 (NCC27) at 1.4-A resolution., Harrop SJ, DeMaere MZ, Fairlie WD, Reztsova T, Valenzuela SM, Mazzanti M, Tonini R, Qiu MR, Jankova L, Warton K, Bauskin AR, Wu WM, Pankhurst S, Campbell TJ, Breit SN, Curmi PM, J Biol Chem. 2001 Nov 30;276(48):44993-5000. Epub 2001 Sep 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11551966 11551966]
+
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Bauskin, A.R.]]
+
[[Category: Bauskin AR]]
-
[[Category: Breit, S.N.]]
+
[[Category: Breit SN]]
-
[[Category: Campbell, T.J.]]
+
[[Category: Campbell TJ]]
-
[[Category: Curmi, P.M.G.]]
+
[[Category: Curmi PMG]]
-
[[Category: DeMaere, M.Z.]]
+
[[Category: DeMaere MZ]]
-
[[Category: Fairlie, W.D.]]
+
[[Category: Fairlie WD]]
-
[[Category: Harrop, S.J.]]
+
[[Category: Harrop SJ]]
-
[[Category: Jankova, L.]]
+
[[Category: Jankova L]]
-
[[Category: Mazzanti, M.]]
+
[[Category: Mazzanti M]]
-
[[Category: Pankhurst, S.]]
+
[[Category: Pankhurst S]]
-
[[Category: Qiu, M.R.]]
+
[[Category: Qiu MR]]
-
[[Category: Reztsova, T.]]
+
[[Category: Reztsova T]]
-
[[Category: Tonini, R.]]
+
[[Category: Tonini R]]
-
[[Category: Valenzuela, S.M.]]
+
[[Category: Valenzuela SM]]
-
[[Category: Warton, K.]]
+
[[Category: Warton K]]
-
[[Category: Wu, W.M.]]
+
[[Category: Wu WM]]
-
[[Category: GTT]]
+
-
[[Category: chloride ion channel]]
+
-
[[Category: clic1]]
+
-
[[Category: glutathione]]
+
-
[[Category: glutathione-s-tranferase superfamily]]
+
-
[[Category: ncc27]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:46:09 2007''
+

Current revision

Chloride Intracellular Channel 1 (CLIC1) complexed with glutathione

PDB ID 1k0n

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1k0n"
Personal tools