1k20

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[[Image:1k20.jpg|left|200px]]<br /><applet load="1k20" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k20, resolution 1.50&Aring;" />
 
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'''Inorganic Pyrophosphatase (family II) from Streptococcus gordonii at 1.5 A resolution'''<br />
 
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==Overview==
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==Inorganic Pyrophosphatase (family II) from Streptococcus gordonii at 1.5 A resolution==
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Recently, a new class of soluble inorganic pyrophosphatase (type-C PPase) has been described that is not homologous in amino acid sequence or kinetic properties to the well-studied PPases (types A and B) found in many organisms from bacteria to humans and thought to be essential to the cell. Structural studies of the type-C PPases from Streptococcus gordonii and Bacillus subtilis reveal a homodimeric structure, with each polypeptide folding into two domains joined by a flexible hinge. The active site, formed at the interface between the N and C-terminal domains, binds two manganese ions approximately 3.6 A apart in a conformation resembling binuclear metal centres found in other hydrolytic enzymes. An activated water molecule bridging the two metal ions is likely poised for nucleophilic attack of the substrate. Importantly, the S. gordonii and B. subtilis enzymes have crystallised in strikingly different conformations. In both subunits of the S. gordonii crystal structure (1.5 A resolution) the C-terminal domain is positioned such that the active site is occluded, with a sulphate ion bound in the active site. In contrast, in the B. subtilis structure (3.0 A resolution) the C-terminal domain is rotated by about 90 degrees, leaving the active site wide open and accessible for substrate binding.
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<StructureSection load='1k20' size='340' side='right'caption='[[1k20]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k20]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K20 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k20 OCA], [https://pdbe.org/1k20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k20 RCSB], [https://www.ebi.ac.uk/pdbsum/1k20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k20 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PPAC_STRGC PPAC_STRGC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k2/1k20_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k20 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1K20 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K20 OCA].
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*[[Inorganic pyrophosphatase 3D structures|Inorganic pyrophosphatase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The "open" and "closed" structures of the type-C inorganic pyrophosphatases from Bacillus subtilis and Streptococcus gordonii., Ahn S, Milner AJ, Futterer K, Konopka M, Ilias M, Young TW, White SA, J Mol Biol. 2001 Nov 2;313(4):797-811. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11697905 11697905]
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[[Category: Large Structures]]
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[[Category: Inorganic diphosphatase]]
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[[Category: Single protein]]
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[[Category: Streptococcus gordonii]]
[[Category: Streptococcus gordonii]]
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[[Category: Ahn, S.]]
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[[Category: Ahn S]]
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[[Category: Futterer, K.]]
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[[Category: Futterer K]]
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[[Category: Ilias, M.]]
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[[Category: Ilias M]]
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[[Category: Konopka, M.]]
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[[Category: Konopka M]]
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[[Category: Milner, A J.]]
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[[Category: Milner AJ]]
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[[Category: White, S A.]]
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[[Category: White SA]]
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[[Category: Young, T W.]]
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[[Category: Young TW]]
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[[Category: GOL]]
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[[Category: MN]]
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[[Category: SO4]]
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[[Category: binuclear metal centre]]
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[[Category: family ii ppase]]
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[[Category: manganese]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:15 2008''
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Current revision

Inorganic Pyrophosphatase (family II) from Streptococcus gordonii at 1.5 A resolution

PDB ID 1k20

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