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1k30

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[[Image:1k30.png|left|200px]]
 
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{{STRUCTURE_1k30| PDB=1k30 | SCENE= }}
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==Crystal Structure Analysis of Squash (Cucurbita moschata) glycerol-3-phosphate (1)-acyltransferase==
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<StructureSection load='1k30' size='340' side='right'caption='[[1k30]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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===Crystal Structure Analysis of Squash (Cucurbita moschata) glycerol-3-phosphate (1)-acyltransferase===
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k30]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cucurbita_moschata Cucurbita moschata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K30 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K30 FirstGlance]. <br>
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{{ABSTRACT_PUBMED_11377195}}
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k30 OCA], [https://pdbe.org/1k30 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k30 RCSB], [https://www.ebi.ac.uk/pdbsum/1k30 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k30 ProSAT]</span></td></tr>
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==About this Structure==
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</table>
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[[1k30]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cucurbita_moschata Cucurbita moschata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K30 OCA].
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== Function ==
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[https://www.uniprot.org/uniprot/GPAT2_CUCMO GPAT2_CUCMO] Esterifies the acyl-group from acyl-acyl carrier proteins (acyl-ACPs) to the sn-1 position of glycerol-3-phosphate (Ref.3). The physiological acyl donors in chloroplasts are acyl-ACPs, but acyl-CoAs are used as artificial donor for in vitro reactions (Probable). The enzyme from chilling-resistant plants discriminates against non-fluid palmitic acid and selects oleic acid whereas the enzyme from sensitive plants accepts both fatty acids (Ref.3). Squash is chilling-sensitive (Probable). Does not seem to discriminate between the acyl-ACP thioesters 18:1-ACP, 18:0-ACP and 16:0-ACP (Ref.3). Exhibits higher selectivity for 16:0-CoA than 18:1-CoA in vitro (PubMed:9016814, PubMed:14684887).<ref>PMID:14684887</ref> <ref>PMID:9016814</ref> <ref>PMID:9016814</ref> <ref>PMID:9016814</ref>
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==Reference==
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== Evolutionary Conservation ==
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<ref group="xtra">PMID:011377195</ref><references group="xtra"/>
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k3/1k30_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k30 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Cucurbita moschata]]
[[Category: Cucurbita moschata]]
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[[Category: Glycerol-3-phosphate O-acyltransferase]]
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[[Category: Large Structures]]
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[[Category: Fawcett, T.]]
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[[Category: Fawcett T]]
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[[Category: Kroon, J T.]]
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[[Category: Kroon JT]]
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[[Category: Murata, N.]]
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[[Category: Murata N]]
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[[Category: Nishida, I.]]
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[[Category: Nishida I]]
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[[Category: Rafferty, J B.]]
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[[Category: Rafferty JB]]
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[[Category: Rice, D W.]]
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[[Category: Rice DW]]
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[[Category: Schierer, T P.]]
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[[Category: Schierer TP]]
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[[Category: Sedelnikova, S E.]]
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[[Category: Sedelnikova SE]]
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[[Category: Simon, J W.]]
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[[Category: Simon JW]]
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[[Category: Slabas, A R.]]
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[[Category: Slabas AR]]
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[[Category: Turnbull, A P.]]
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[[Category: Turnbull AP]]
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[[Category: Four-helix bundle]]
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[[Category: Transferase]]
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Current revision

Crystal Structure Analysis of Squash (Cucurbita moschata) glycerol-3-phosphate (1)-acyltransferase

PDB ID 1k30

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