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1kgd

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[[Image:1kgd.jpg|left|200px]]<br /><applet load="1kgd" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kgd, resolution 1.314&Aring;" />
 
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'''Crystal Structure of the Guanylate Kinase-like Domain of Human CASK'''<br />
 
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==Overview==
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==Crystal Structure of the Guanylate Kinase-like Domain of Human CASK==
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CASK is a member of the membrane-associated guanylate kinases (MAGUK), homologs, a family of proteins that scaffold protein complexes at, particular regions of the plasma membrane by utilizing multiple, protein-binding domains. The GK domain of MAGUKs, which shares high, similarity in amino acid sequence with yeast guanylate kinase (yGMPK), is, the least characterized MAGUK domain both in structure and function. In, addition to its scaffolding function, the GK domain of hCASK has been, shown to be involved in transcription regulation. Here we report the, crystal structure of the GK domain of human CASK (hCASK-GK) at 1.3-A, resolution. The structure rationalizes the inability of the GK domain to, catalyze phosphoryl transfer and strongly supports its new function as a, protein-binding module. Comparison of the hCASK-GK structure with the, available crystal structures of yGMPK provides insight into possible, conformational changes that occur in hCASK upon GMP binding. These, conformational changes may act to regulate hCASK-GK function in a, nucleotide-dependent manner.
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<StructureSection load='1kgd' size='340' side='right'caption='[[1kgd]], [[Resolution|resolution]] 1.31&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1kgd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KGD FirstGlance]. <br>
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1KGD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KGD OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.314&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kgd OCA], [https://pdbe.org/1kgd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kgd RCSB], [https://www.ebi.ac.uk/pdbsum/1kgd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kgd ProSAT]</span></td></tr>
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Structural basis for nucleotide-dependent regulation of membrane-associated guanylate kinase-like domains., Li Y, Spangenberg O, Paarmann I, Konrad M, Lavie A, J Biol Chem. 2002 Feb 8;277(6):4159-65. Epub 2001 Nov 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11729206 11729206]
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/CSKP_HUMAN CSKP_HUMAN] Defects in CASK are the cause of mental retardation and microcephaly with pontine and cerebellar hypoplasia (MICPCH) [MIM:[https://omim.org/entry/300749 300749]. A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Patients with mental retardation X-linked CASK-related can manifest a severe phenotype consisting of severe intellectual deficit, congenital or postnatal microcephaly, disproportionate brainstem and cerebellar hypoplasia. A milder phenotype consists of mental retardation alone or associated with nystagmus.<ref>PMID:19165920</ref> Defects in CASK are the cause of FG syndrome type 4 (FGS4) [MIM:[https://omim.org/entry/300422 300422]. FG syndrome (FGS) is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.<ref>PMID:19200522</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/CSKP_HUMAN CSKP_HUMAN] Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kg/1kgd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kgd ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Konrad, M.]]
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[[Category: Konrad M]]
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[[Category: Lavie, A.]]
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[[Category: Lavie A]]
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[[Category: Li, Y.]]
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[[Category: Li Y]]
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[[Category: Paarmann, I.]]
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[[Category: Paarmann I]]
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[[Category: Spangenberg, O.]]
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[[Category: Spangenberg O]]
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[[Category: FMT]]
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[[Category: cask]]
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[[Category: guanylate kinase like domain]]
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[[Category: maguk]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:13:20 2008''
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Current revision

Crystal Structure of the Guanylate Kinase-like Domain of Human CASK

PDB ID 1kgd

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