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1oa0

From Proteopedia

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[[Image:1oa0.jpg|left|200px]]<br /><applet load="1oa0" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1oa0, resolution 1.25&Aring;" />
 
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'''REDUCED HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO DESULFURICANS X-RAY STRUCTURE AT 1.25A RESOLUTION'''<br />
 
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==Overview==
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==REDUCED HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO DESULFURICANS X-RAY STRUCTURE AT 1.25A RESOLUTION==
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The hybrid cluster proteins from the sulfate reducing bacteria Desulfovibrio desulfuricans ATCC 27774 ( Dd) and Desulfovibrio vulgaris strain Hildenborough ( Dv) have been isolated and crystallized anaerobically. In each case, the protein has been reduced with dithionite and the crystal structure of the reduced form elucidated using X-ray synchrotron radiation techniques at 1.25 A and 1.55 A resolution for Dd and Dv, respectively. Although the overall structures of the proteins are unchanged upon reduction, there are significant changes at the hybrid cluster centres. These include significant movements in the position of the iron atom linked to the persulfide moiety in the oxidized as-isolated proteins and the sulfur atom of the persulfide itself. The nature of these changes is described and the implications with respect to the function of hybrid cluster proteins are discussed.
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<StructureSection load='1oa0' size='340' side='right'caption='[[1oa0]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1oa0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OA0 FirstGlance]. <br>
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1OA0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=SF3:'>SF3</scene> and <scene name='pdbligand=MES:'>MES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mes+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OA0 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SF3:FE4-S3+CLUSTER'>SF3</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oa0 OCA], [https://pdbe.org/1oa0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oa0 RCSB], [https://www.ebi.ac.uk/pdbsum/1oa0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oa0 ProSAT]</span></td></tr>
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Reduced hybrid cluster proteins (HCP) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at high resolution using synchrotron radiation., Aragao D, Macedo S, Mitchell EP, Romao CV, Liu MY, Frazao C, Saraiva LM, Xavier AV, LeGall J, van Dongen WM, Hagen WR, Teixeira M, Carrondo MA, Lindley P, J Biol Inorg Chem. 2003 May;8(5):540-8. Epub 2003 Feb 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12764602 12764602]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HCP_DESDA HCP_DESDA] Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O (By similarity).[HAMAP-Rule:MF_00069]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/1oa0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oa0 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Desulfovibrio desulfuricans]]
[[Category: Desulfovibrio desulfuricans]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Aragao, D.]]
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[[Category: Aragao D]]
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[[Category: Carrondo, M A.]]
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[[Category: Carrondo MA]]
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[[Category: Dongen, W M.A M.Van.]]
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[[Category: Frazao C]]
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[[Category: Frazao, C.]]
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[[Category: Hagen WR]]
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[[Category: Hagen, W R.]]
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[[Category: Legall J]]
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[[Category: Legall, J.]]
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[[Category: Lindley PF]]
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[[Category: Lindley, P F.]]
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[[Category: Liu MY]]
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[[Category: Liu, M Y.]]
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[[Category: Macedo S]]
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[[Category: Macedo, S.]]
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[[Category: Mitchell EP]]
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[[Category: Mitchell, E P.]]
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[[Category: Romao CV]]
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[[Category: Romao, C V.]]
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[[Category: Saraiva LM]]
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[[Category: Saraiva, L M.]]
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[[Category: Teixeira M]]
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[[Category: Teixeira, M.]]
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[[Category: Van Dongen WMAM]]
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[[Category: Xavier, A V.]]
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[[Category: Xavier AV]]
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[[Category: MES]]
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[[Category: SF3]]
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[[Category: SF4]]
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[[Category: desulfovibrio desulfuricans]]
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[[Category: high-resolution]]
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[[Category: hybrid cluster]]
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[[Category: reduced forms]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:15:11 2008''
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Current revision

REDUCED HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO DESULFURICANS X-RAY STRUCTURE AT 1.25A RESOLUTION

PDB ID 1oa0

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