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5oa5

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(New page: ==CELLOBIOHYDROLASE I (CEL7A) FROM HYPOCREA JECORINA WITH IMPROVED THERMAL STABILITY== <StructureSection load='5oa5' size='340' side='right' caption='5oa5, resolution 2...)
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==CELLOBIOHYDROLASE I (CEL7A) FROM HYPOCREA JECORINA WITH IMPROVED THERMAL STABILITY==
==CELLOBIOHYDROLASE I (CEL7A) FROM HYPOCREA JECORINA WITH IMPROVED THERMAL STABILITY==
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<StructureSection load='5oa5' size='340' side='right' caption='[[5oa5]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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<StructureSection load='5oa5' size='340' side='right'caption='[[5oa5]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5oa5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OA5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OA5 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5oa5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OA5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OA5 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oa5 OCA], [https://pdbe.org/5oa5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oa5 RCSB], [https://www.ebi.ac.uk/pdbsum/5oa5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oa5 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oa5 OCA], [http://pdbe.org/5oa5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oa5 RCSB], [http://www.ebi.ac.uk/pdbsum/5oa5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oa5 ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/GUX1_HYPJE GUX1_HYPJE]] The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
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[https://www.uniprot.org/uniprot/GUX1_HYPJE GUX1_HYPJE] The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Secreted mixtures of Hypocrea jecorina cellulases are able to efficiently degrade cellulosic biomass to fermentable sugars at large, commercially relevant scales. H. jecorina Cel7A, cellobiohydrolase I, from glycoside hydrolase family 7, is the workhorse enzyme of the process. However, the thermal stability of Cel7A limits its use to processes where temperatures are no higher than 50 degrees C. Enhanced thermal stability is desirable to enable use of higher process temperatures and improve the economic feasibility of industrial biomass conversion. Here, we have enhanced the thermal stability of Cel7A through directed evolution. Sites with increased thermal stability properties were combined, and a Cel7A variant (FCA398) was obtained, which has a 10.4 degrees C increase in Tm and a 44-fold greater half-life compared to the wild type enzyme. This Cel7A variant contains 18 mutated sites and is active under application conditions up to at least 75 degrees C. The X-ray crystal structure of the catalytic domain was determined at 2.1 A resolution and shows that the effects of the mutations are local and do not introduce major backbone conformational changes. Molecular dynamics simulations reveal the catalytic domain of wild type Cel7A and the FCA398 variant exhibit similar behavior at 300 K, while at elevated temperature (475 K and 525 K), the FCA398 variant fluctuates less and maintains more native contacts over time. Combining the structural and dynamic investigations, rationales were developed for the stabilizing effect at many of the mutated sites.
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Improving the thermal stability of cellobiohydrolase Cel7A from Hypocrea jecorina by directed evolution.,Goedegebuur F, Dankmeyer L, Gualfetti P, Karkehabadi S, Hansson H, Jana S, Huynh V, Kelemen BR, Kruithof P, Larenas EA, Teunissen PJM, Stahlberg J, Payne CM, Mitchinson C, Sandgren M J Biol Chem. 2017 Aug 31. pii: jbc.M117.803270. doi: 10.1074/jbc.M117.803270. PMID:28860192<ref>PMID:28860192</ref>
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==See Also==
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*[[Cellobiohydrolase 3D structures|Cellobiohydrolase 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5oa5" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Goedegebuur, F]]
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[[Category: Large Structures]]
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[[Category: Hansson, H]]
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[[Category: Trichoderma reesei]]
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[[Category: Karkehabadi, S]]
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[[Category: Goedegebuur F]]
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[[Category: Mikkelsen, N]]
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[[Category: Hansson H]]
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[[Category: Sandgren, M]]
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[[Category: Karkehabadi S]]
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[[Category: Stahlberg, J]]
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[[Category: Mikkelsen N]]
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[[Category: Cellulase]]
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[[Category: Sandgren M]]
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[[Category: Hydrolase]]
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[[Category: Stahlberg J]]
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[[Category: Protein engineering]]
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Current revision

CELLOBIOHYDROLASE I (CEL7A) FROM HYPOCREA JECORINA WITH IMPROVED THERMAL STABILITY

PDB ID 5oa5

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