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3j9g

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==Atomic model of the VipA/VipB, the type six secretion system contractile sheath of Vibrio cholerae from cryo-EM==
==Atomic model of the VipA/VipB, the type six secretion system contractile sheath of Vibrio cholerae from cryo-EM==
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<StructureSection load='3j9g' size='340' side='right' caption='[[3j9g]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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<SX load='3j9g' size='340' side='right' viewer='molstar' caption='[[3j9g]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3j9g]] is a 60 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J9G FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3j9g]] is a 60 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J9G FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j9g RCSB], [http://www.ebi.ac.uk/pdbsum/3j9g PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9g OCA], [https://pdbe.org/3j9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j9g RCSB], [https://www.ebi.ac.uk/pdbsum/3j9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9g ProSAT]</span></td></tr>
</table>
</table>
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{{Large structure}}
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/Q9KN58_VIBCH Q9KN58_VIBCH]
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== Publication Abstract from PubMed ==
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Bacteria use rapid contraction of a long sheath of the type VI secretion system (T6SS) to deliver effectors into a target cell. Here, we present an atomic-resolution structure of a native contracted Vibrio cholerae sheath determined by cryo-electron microscopy. The sheath subunits, composed of tightly interacting proteins VipA and VipB, assemble into a six-start helix. The helix is stabilized by a core domain assembled from four beta strands donated by one VipA and two VipB molecules. The fold of inner and middle layers is conserved between T6SS and phage sheaths. However, the structure of the outer layer is distinct and suggests a mechanism of interaction of the bacterial sheath with an accessory ATPase, ClpV, that facilitates multiple rounds of effector delivery. Our results provide a mechanistic insight into assembly of contractile nanomachines that bacteria and phages use to translocate macromolecules across membranes.
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Structure of the Type VI Secretion System Contractile Sheath.,Kudryashev M, Wang RY, Brackmann M, Scherer S, Maier T, Baker D, DiMaio F, Stahlberg H, Egelman EH, Basler M Cell. 2015 Feb 26;160(5):952-62. doi: 10.1016/j.cell.2015.01.037. PMID:25723169<ref>PMID:25723169</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Baker, D]]
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[[Category: Large Structures]]
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[[Category: Basler, M]]
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[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
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[[Category: Brackmann, M]]
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[[Category: Baker D]]
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[[Category: DiMaio, F]]
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[[Category: Basler M]]
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[[Category: Egelman, E H]]
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[[Category: Brackmann M]]
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[[Category: Kudryashev, M]]
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[[Category: DiMaio F]]
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[[Category: Maier, T]]
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[[Category: Egelman EH]]
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[[Category: Scherer, S]]
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[[Category: Kudryashev M]]
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[[Category: Stahlberg, H]]
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[[Category: Maier T]]
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[[Category: Wang, R Y.R]]
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[[Category: Scherer S]]
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[[Category: Bacterial secretion]]
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[[Category: Stahlberg H]]
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[[Category: Contractile protein]]
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[[Category: Wang RY-R]]
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[[Category: Contraction]]
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[[Category: Phage]]
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[[Category: T6ss]]
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Current revision

Atomic model of the VipA/VipB, the type six secretion system contractile sheath of Vibrio cholerae from cryo-EM

3j9g, resolution 3.50Å

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