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3j9x

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==A Virus that Infects a Hyperthermophile Encapsidates A-Form DNA==
==A Virus that Infects a Hyperthermophile Encapsidates A-Form DNA==
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<StructureSection load='3j9x' size='340' side='right' caption='[[3j9x]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
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<SX load='3j9x' size='340' side='right' viewer='molstar' caption='[[3j9x]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3j9x]] is a 60 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_islandicus_rod-shaped_virus_2 Sulfolobus islandicus rod-shaped virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J9X FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3j9x]] is a 60 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_islandicus_rod-shaped_virus_2 Sulfolobus islandicus rod-shaped virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J9X FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9x OCA], [http://pdbe.org/3j9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j9x RCSB], [http://www.ebi.ac.uk/pdbsum/3j9x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9x ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9x OCA], [https://pdbe.org/3j9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j9x RCSB], [https://www.ebi.ac.uk/pdbsum/3j9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9x ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/CAPSD_SIRV2 CAPSD_SIRV2] Self-assembles to form a helical, filamentous nucleocapsid (PubMed:25999507). The capsid proteins wrap around the DNA and maintain it in an A-form by non-specific desolvation and specific coordination of the DNA phosphate groups by positively charged residues (Probable) (PubMed:25999507). This certainly protects the viral DNA under conditions such as the extreme desiccation of its host (Probable).<ref>PMID:25999507</ref> <ref>PMID:25999507</ref> <ref>PMID:32759221</ref>
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Extremophiles, microorganisms thriving in extreme environmental conditions, must have proteins and nucleic acids that are stable at extremes of temperature and pH. The nonenveloped, rod-shaped virus SIRV2 (Sulfolobus islandicus rod-shaped virus 2) infects the hyperthermophilic acidophile Sulfolobus islandicus, which lives at 80 degrees C and pH 3. We have used cryo-electron microscopy to generate a three-dimensional reconstruction of the SIRV2 virion at ~4 angstrom resolution, which revealed a previously unknown form of virion organization. Although almost half of the capsid protein is unstructured in solution, this unstructured region folds in the virion into a single extended alpha helix that wraps around the DNA. The DNA is entirely in the A-form, which suggests a common mechanism with bacterial spores for protecting DNA in the most adverse environments.
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Virology. A virus that infects a hyperthermophile encapsidates A-form DNA.,DiMaio F, Yu X, Rensen E, Krupovic M, Prangishvili D, Egelman EH Science. 2015 May 22;348(6237):914-7. doi: 10.1126/science.aaa4181. PMID:25999507<ref>PMID:25999507</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3j9x" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Virus coat protein|Virus coat protein]]
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Large Structures]]
[[Category: Sulfolobus islandicus rod-shaped virus 2]]
[[Category: Sulfolobus islandicus rod-shaped virus 2]]
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[[Category: DiMaio, F]]
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[[Category: DiMaio F]]
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[[Category: Egelman, E]]
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[[Category: Egelman E]]
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[[Category: Krupovic, M]]
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[[Category: Krupovic M]]
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[[Category: Prangishvili, D]]
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[[Category: Prangishvili D]]
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[[Category: Rensen, E]]
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[[Category: Rensen E]]
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[[Category: Yu, X]]
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[[Category: Yu X]]
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[[Category: Archaeal virus]]
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[[Category: Helical symmetry]]
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[[Category: Virus]]
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Current revision

A Virus that Infects a Hyperthermophile Encapsidates A-Form DNA

3j9x, resolution 3.80Å

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