3mei

<StructureSection load='3mei' size='340' side='right' caption='[[3mei]], [[Resolution|resolution]] 1.97&Aring;' scene=''>

<table><tr><td colspan='2'>[[3mei]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MEI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MEI FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>

<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5BU:5-BROMO-URIDINE-5-MONOPHOSPHATE'>5BU</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mei OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mei RCSB], [http://www.ebi.ac.uk/pdbsum/3mei PDBsum]</span></td></tr>

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== Publication Abstract from PubMed ==

A sequence around the start codon of the mRNA of human thymidylate synthase (TS) folds into a secondary-structure motif in which the initiation site is sequestered in a metastable hairpin. Binding of the protein to its own mRNA at the hairpin prevents the production of TS through a translation-repression feedback mechanism. Stabilization of the mRNA hairpin by other ligands has been proposed as a strategy to reduce TS levels in anticancer therapy. Rapidly proliferating cells require high TS activity to maintain the production of thymidine as a building block for DNA synthesis. The crystal structure of a model oligonucleotide (TS1) that represents the TS-binding site of the mRNA has been determined. While fluorescence studies showed that the TS1 RNA preferentially adopts a hairpin structure in solution, even at high RNA concentrations, an asymmetric dimer of two hybridized TS1 strands was obtained in the crystal. The TS1 dimer contains an unusual S-turn motif that also occurs in the `off' state of the human ribosomal decoding site RNA.

Structure of an RNA dimer of a regulatory element from human thymidylate synthase mRNA.,Dibrov S, McLean J, Hermann T Acta Crystallogr D Biol Crystallogr. 2011 Feb;67(Pt 2):97-104. Epub 2011, Jan 8. PMID:21245530<ref>PMID:21245530</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

<references/>

[[Category: Dibrov, S]]

[[Category: Hermann, T]]

[[Category: McLean, J]]

[[Category: C-c base pair]]

[[Category: S-turn]]