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1q5f
From Proteopedia
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'''NMR Structure of Type IVb pilin (PilS) from Salmonella typhi''' | '''NMR Structure of Type IVb pilin (PilS) from Salmonella typhi''' | ||
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[[Category: Xu, X F.]] | [[Category: Xu, X F.]] | ||
[[Category: Zhang, M.]] | [[Category: Zhang, M.]] | ||
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Revision as of 02:53, 3 May 2008
NMR Structure of Type IVb pilin (PilS) from Salmonella typhi
Overview
The structure of the N-terminal-truncated Type IVb structural pilin (t-PilS) from Salmonella typhi was determined by NMR. Although topologically similar to the recently determined x-ray structure of pilin from Vibrio cholerae toxin-coregulated pilus, the only Type IVb pilin with known structure, t-PilS contains many distinct structural features. The protein contains an extra pair of beta-strands in the N-terminal alphabeta loop that align with the major beta-strands to form a continuous 7-stranded antiparallel beta-sheet. The C-terminal disulfide-bonded region of t-PilS is only half the length of that of toxin-coregulated pilus pilin. A model of S. typhi pilus has been proposed and mutagenesis studies suggested that residues on both the alphabeta loop and the C-terminal disulfide-bonded region of PilS might be involved in binding specificity of the pilus. This model structure reveals an exposed surface between adjacent subunits of PilS that could be a potential binding site for the cystic fibrosis transmembrane conductance regulator.
About this Structure
1Q5F is a Single protein structure of sequence from Salmonella typhi. Full crystallographic information is available from OCA.
Reference
NMR structure of a type IVb pilin from Salmonella typhi and its assembly into pilus., Xu XF, Tan YW, Lam L, Hackett J, Zhang M, Mok YK, J Biol Chem. 2004 Jul 23;279(30):31599-605. Epub 2004 May 24. PMID:15159389 Page seeded by OCA on Sat May 3 05:53:07 2008
