This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1kpb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:26, 14 February 2024) (edit) (undo)
 
(19 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1kpb.gif|left|200px]]<br />
 
-
<applet load="1kpb" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1kpb, resolution 2.0&Aring;" />
 
-
'''PKCI-1-APO'''<br />
 
-
==Overview==
+
==PKCI-1-APO==
-
The three-dimensional structure of protein kinase C interacting protein 1, (PKCI-1) has been solved to high resolution by x-ray crystallography using, single isomorphous replacement with anomalous scattering. The gene, encoding human PKCI-1 was cloned from a cDNA library by using a partial, sequence obtained from interactions identified in the yeast two-hybrid, system between PKCI-1 and the regulatory domain of protein kinase C-beta., The PKCI-1 protein was expressed in Pichia pastoris as a dimer of two, 13.7-kDa polypeptides. PKCI-1 is a member of the HIT family of proteins, shown by sequence identity to be conserved in a broad range of organisms, including mycoplasma, plants, and humans. Despite the ubiquity of this, protein sequence in nature, no distinct function has been shown for ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8643579 (full description)]]
+
<StructureSection load='1kpb' size='340' side='right'caption='[[1kpb]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1kpb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KPB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KPB FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kpb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kpb OCA], [https://pdbe.org/1kpb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kpb RCSB], [https://www.ebi.ac.uk/pdbsum/1kpb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kpb ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/HINT1_HUMAN HINT1_HUMAN] Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2 (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kp/1kpb_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kpb ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1KPB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]]. Structure known Active Sites: ZNA and ZNB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KPB OCA]].
+
*[[Histidine triad nucleotide-binding protein 3D structures|Histidine triad nucleotide-binding protein 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins., Lima CD, Klein MG, Weinstein IB, Hendrickson WA, Proc Natl Acad Sci U S A. 1996 May 28;93(11):5357-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8643579 8643579]
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Hendrickson, W.A.]]
+
[[Category: Hendrickson WA]]
-
[[Category: Klein, M.G.]]
+
[[Category: Klein MG]]
-
[[Category: Lima, C.D.]]
+
[[Category: Lima CD]]
-
[[Category: Weinstein, I.B.]]
+
[[Category: Weinstein IB]]
-
[[Category: pkc]]
+
-
[[Category: pkci-1]]
+
-
[[Category: signal transduction]]
+
-
[[Category: zinc binding protein]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:41:28 2007''
+

Current revision

PKCI-1-APO

PDB ID 1kpb

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1kpb"
Personal tools