1kq1

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[[Image:1kq1.gif|left|200px]]
 
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{{Structure
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==1.55 A Crystal structure of the pleiotropic translational regulator, Hfq==
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|PDB= 1kq1 |SIZE=350|CAPTION= <scene name='initialview01'>1kq1</scene>, resolution 1.55&Aring;
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<StructureSection load='1kq1' size='340' side='right'caption='[[1kq1]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>
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<table><tr><td colspan='2'>[[1kq1]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KQ1 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kq1 OCA], [https://pdbe.org/1kq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kq1 RCSB], [https://www.ebi.ac.uk/pdbsum/1kq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kq1 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1kq2|1KQ2]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kq1 OCA], [http://www.ebi.ac.uk/pdbsum/1kq1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kq1 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/A0A0H3JV59_STAAM A0A0H3JV59_STAAM] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs.[HAMAP-Rule:MF_00436]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kq/1kq1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kq1 ConSurf].
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<div style="clear:both"></div>
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'''1.55 A Crystal structure of the pleiotropic translational regulator, Hfq'''
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==See Also==
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*[[Protein Hfq 3D structures|Protein Hfq 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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In prokaryotes, Hfq regulates translation by modulating the structure of numerous RNA molecules by binding preferentially to A/U-rich sequences. To elucidate the mechanisms of target recognition and translation regulation by Hfq, we determined the crystal structures of the Staphylococcus aureus Hfq and an Hfq-RNA complex to 1.55 and 2.71 A resolution, respectively. The structures reveal that Hfq possesses the Sm-fold previously observed only in eukaryotes and archaea. However, unlike these heptameric Sm proteins, Hfq forms a homo-hexameric ring. The Hfq-RNA structure reveals that the single-stranded hepta-oligoribonucleotide binds in a circular conformation around a central basic cleft, whereby Tyr42 residues from adjacent subunits stack with six of the bases, and Gln8, outside the Sm motif, provides key protein-base contacts. Such binding suggests a mechanism for Hfq function.
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[[Category: Large Structures]]
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==About this Structure==
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1KQ1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KQ1 OCA].
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==Reference==
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Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein., Schumacher MA, Pearson RF, Moller T, Valentin-Hansen P, Brennan RG, EMBO J. 2002 Jul 1;21(13):3546-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12093755 12093755]
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Brennan, R G.]]
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[[Category: Brennan RG]]
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[[Category: Moller, T.]]
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[[Category: Moller T]]
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[[Category: Pearson, R F.]]
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[[Category: Pearson RF]]
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[[Category: Schumacher, M A.]]
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[[Category: Schumacher MA]]
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[[Category: Valentin-Hansen, P.]]
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[[Category: Valentin-Hansen P]]
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[[Category: hexamer]]
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[[Category: hfq]]
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[[Category: rna binding protein]]
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[[Category: sm motif]]
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[[Category: translational regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:52:01 2008''
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Current revision

1.55 A Crystal structure of the pleiotropic translational regulator, Hfq

PDB ID 1kq1

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