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1ku3

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(New page: 200px<br /><applet load="1ku3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ku3, resolution 1.8&Aring;" /> '''Crystal Structure of ...)
Current revision (07:27, 14 February 2024) (edit) (undo)
 
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[[Image:1ku3.gif|left|200px]]<br /><applet load="1ku3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ku3, resolution 1.8&Aring;" />
 
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'''Crystal Structure of Thermus aquaticus RNA Polymerase Sigma Subunit Fragment, Region 4'''<br />
 
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==Overview==
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==Crystal Structure of Thermus aquaticus RNA Polymerase Sigma Subunit Fragment, Region 4==
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The sigma subunit is the key regulator of bacterial transcription., Proteolysis of Thermus aquaticus sigma(A), which occurred in situ during, crystallization, reveals three domains, sigma(2), sigma(3), and sigma(4), connected by flexible linkers. Crystal structures of each domain were, determined, as well as of sigma(4) complexed with -35 element DNA. Exposed, surfaces of each domain are important for RNA polymerase binding., Universally conserved residues important for -10 element recognition and, melting lie on one face of sigma(2), while residues important for extended, -10 recognition lie on sigma(3). Genetic studies correctly predicted that, a helix-turn-helix motif in sigma(4) recognizes the -35 element but not, the details of the protein-DNA interactions. Positive control mutants in, sigma(4) cluster in two regions, positioned to interact with activators, bound just upstream or downstream of the -35 element.
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<StructureSection load='1ku3' size='340' side='right'caption='[[1ku3]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ku3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KU3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ku3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ku3 OCA], [https://pdbe.org/1ku3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ku3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ku3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ku3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SIGA_THEAQ SIGA_THEAQ] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.[HAMAP-Rule:MF_00963]<ref>PMID:11114902</ref> <ref>PMID:11931761</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/1ku3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ku3 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1KU3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KU3 OCA].
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of the bacterial RNA polymerase promoter specificity sigma subunit., Campbell EA, Muzzin O, Chlenov M, Sun JL, Olson CA, Weinman O, Trester-Zedlitz ML, Darst SA, Mol Cell. 2002 Mar;9(3):527-39. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11931761 11931761]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
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[[Category: Campbell, E.A.]]
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[[Category: Campbell EA]]
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[[Category: Chlenov, M.]]
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[[Category: Chlenov M]]
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[[Category: Darst, S.A.]]
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[[Category: Darst SA]]
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[[Category: Muzzin, O.]]
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[[Category: Muzzin O]]
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[[Category: Olson, C.A.]]
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[[Category: Olson CA]]
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[[Category: Sun, J.L.]]
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[[Category: Sun JL]]
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[[Category: Trester-Zedlitz, M.L.]]
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[[Category: Trester-Zedlitz ML]]
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[[Category: Weinman, O.]]
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[[Category: Weinman O]]
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[[Category: helix-turn-helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:57:12 2007''
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Current revision

Crystal Structure of Thermus aquaticus RNA Polymerase Sigma Subunit Fragment, Region 4

PDB ID 1ku3

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