1kyz

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(New page: 200px<br /><applet load="1kyz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kyz, resolution 2.20&Aring;" /> '''Crystal Structure An...)
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[[Image:1kyz.jpg|left|200px]]<br /><applet load="1kyz" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kyz, resolution 2.20&Aring;" />
 
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'''Crystal Structure Analysis of Caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase Ferulic Acid Complex'''<br />
 
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==Overview==
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==Crystal Structure Analysis of Caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase Ferulic Acid Complex==
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Caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase (COMT) from, alfalfa is an S-adenosyl-L-Met-dependent O-methyltransferase involved in, lignin biosynthesis. COMT methylates caffeoyl- and, 5-hydroxyferuloyl-containing acids, aldehydes, and alcohols in vitro while, displaying a kinetic preference for the alcohols and aldehydes over the, free acids. The 2.2-A crystal structure of COMT in complex with, S-adenosyl-L-homocysteine (SAH) and ferulic acid (ferulate form), as well, as the 2.4-A crystal structure of COMT in complex with SAH and, 5-hydroxyconiferaldehyde, provide a structural understanding of the, observed substrate preferences. These crystal structures identify residues, lining the active site surface that contact the substrates. Structurally, guided site-directed mutagenesis of active site residues was performed, with the goal of altering the kinetic preferences for physiological, substrates. The kinetic parameters of the COMT mutants versus wild-type, enzyme are presented, and coupled with the high-resolution crystal, structures, they will serve as a starting point for the in vivo, manipulation of lignin monomers in transgenic plants. Ultimately, this, structurally based approach to metabolic engineering will allow the, further alteration of the lignin biosynthetic pathway in agronomically, important plants. This approach will lead to a better understanding of the, in vivo operation of the potential metabolic grid for monolignol, biosynthesis.
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<StructureSection load='1kyz' size='340' side='right'caption='[[1kyz]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1kyz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KYZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KYZ FirstGlance]. <br>
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1KYZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa] with SAH and FER as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Caffeate_O-methyltransferase Caffeate O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.68 2.1.1.68] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KYZ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FER:3-(4-HYDROXY-3-METHOXYPHENYL)-2-PROPENOIC+ACID'>FER</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kyz OCA], [https://pdbe.org/1kyz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kyz RCSB], [https://www.ebi.ac.uk/pdbsum/1kyz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kyz ProSAT]</span></td></tr>
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Structural basis for the modulation of lignin monomer methylation by caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase., Zubieta C, Kota P, Ferrer JL, Dixon RA, Noel JP, Plant Cell. 2002 Jun;14(6):1265-77. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12084826 12084826]
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</table>
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[[Category: Caffeate O-methyltransferase]]
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== Function ==
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[https://www.uniprot.org/uniprot/COMT1_MEDSA COMT1_MEDSA] Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid. The resulting products may subsequently be converted to the corresponding alcohols that are incorporated into lignins.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ky/1kyz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kyz ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Medicago sativa]]
[[Category: Medicago sativa]]
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[[Category: Single protein]]
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[[Category: Dixon RA]]
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[[Category: Dixon, R.A.]]
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[[Category: Ferrer J-L]]
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[[Category: Ferrer, J.L.]]
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[[Category: Kota P]]
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[[Category: Kota, P.]]
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[[Category: Noel JP]]
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[[Category: Noel, J.P.]]
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[[Category: Zubieta C]]
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[[Category: Zubieta, C.]]
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[[Category: FER]]
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[[Category: SAH]]
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[[Category: ferulic acid]]
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[[Category: lignin]]
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[[Category: methylation]]
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[[Category: o-methyltransferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:05:00 2007''
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Current revision

Crystal Structure Analysis of Caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase Ferulic Acid Complex

PDB ID 1kyz

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