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1l2t

From Proteopedia

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[[Image:1l2t.gif|left|200px]]
 
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{{Structure
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==Dimeric Structure of MJ0796, a Bacterial ABC Transporter Cassette==
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|PDB= 1l2t |SIZE=350|CAPTION= <scene name='initialview01'>1l2t</scene>, resolution 1.90&Aring;
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<StructureSection load='1l2t' size='340' side='right'caption='[[1l2t]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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<table><tr><td colspan='2'>[[1l2t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L2T FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l2t OCA], [https://pdbe.org/1l2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l2t RCSB], [https://www.ebi.ac.uk/pdbsum/1l2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l2t ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l2t OCA], [http://www.ebi.ac.uk/pdbsum/1l2t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1l2t RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Y796_METJA Y796_METJA]
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== Evolutionary Conservation ==
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'''Dimeric Structure of MJ0796, a Bacterial ABC Transporter Cassette'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l2/1l2t_consurf.spt"</scriptWhenChecked>
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It has been proposed that the reaction cycle of ATP binding cassette (ABC) transporters is driven by dimerization of their ABC motor domains upon binding ATP at their mutual interface. However, no such ATP sandwich complex has been observed for an ABC from an ABC transporter. In this paper, we report the crystal structure of a stable dimer formed by the E171Q mutant of the MJ0796 ABC, which is hydrolytically inactive due to mutation of the catalytic base. The structure shows a symmetrical dimer in which two ATP molecules are each sandwiched between the Walker A motif in one subunit and the LSGGQ signature motif in the other subunit. These results establish the stereochemical basis of the power stroke of ABC transporter pumps.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1L2T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2T OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l2t ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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ATP binding to the motor domain from an ABC transporter drives formation of a nucleotide sandwich dimer., Smith PC, Karpowich N, Millen L, Moody JE, Rosen J, Thomas PJ, Hunt JF, Mol Cell. 2002 Jul;10(1):139-49. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12150914 12150914]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
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[[Category: Single protein]]
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[[Category: Hunt JF]]
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[[Category: Hunt, J F.]]
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[[Category: Karpowich N]]
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[[Category: Karpowich, N.]]
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[[Category: Rosen J]]
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[[Category: Rosen, J.]]
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[[Category: Smith PC]]
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[[Category: Smith, P C.]]
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[[Category: abc transporter]]
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[[Category: atpase]]
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[[Category: nbd]]
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[[Category: walker-a]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:57:09 2008''
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Current revision

Dimeric Structure of MJ0796, a Bacterial ABC Transporter Cassette

PDB ID 1l2t

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